TY - JOUR
AU1 - Alimolaei, Mojtaba
AU2 - Golchin, Mehdi
AU3 - Abshenas, Jalil
AU4 - Ezatkhah, Majid
AU5 - Bafti, Mehrdad
AB - The alpha-toxin is one of the virulence factors of Clostridium perfringens for gas gangrene in humans and animals or necrotic enteritis in poultry. The C-terminal domain of this toxin (
cpa
247-370
) was synthesized and cloned into pT1NX vector to construct the pT1NX-alpha plasmid. This surface-expressing plasmid was electroporated into Lactobacillus casei ATCC 393, generating the recombinant L. casei strain expressing alpha-toxoid (LC-α strain). Expression of this modified alpha-toxoid was confirmed by SDS-PAGE, immunoblotting, and direct immunofluorescence microscopy. BALB/c mice, immunized orally by the recombinant LC-α strain, elicited mucosal and significantly humoral immune responses (p < 0.05) and developed a protection against 900 MLD/mL of the standard alpha-toxin. This study showed that this recombinant LC-α strain could be a promising vaccine candidate against gas gangrene and necrotic enteritis.
TI - A Recombinant Probiotic, Lactobacillus casei, Expressing the Clostridium perfringens α-toxoid, as an Orally Vaccine Candidate Against Gas Gangrene and Necrotic Enteritis
JF - Probiotics and Antimicrobial Proteins
DO - 10.1007/s12602-017-9276-8
DA - 2017-04-11
UR - https://www.deepdyve.com/lp/springer-journals/a-recombinant-probiotic-lactobacillus-casei-expressing-the-clostridium-DH71mekoZ6
SP - 251
EP - 257
VL - 10
IS - 2
DP - DeepDyve