TY - JOUR AU1 - Linehan, Sheena A. AU2 - Martínez-Pomares, Luisa AU3 - Stahl, Philip D. AU4 - Gordon, Siamon AB - The mannose receptor (MR) has established roles in macrophage (Mφ) phagocytosis of microorganisms and endocytic clearance of host-derived glycoproteins, and has recently been implicated in antigen capture by dendritic cells (DCs) in vitro. MR is the founder member of a family of homologous proteins, and its recognition properties differ according to its tissue of origin. Given this heterogeneity and our recent discovery of a soluble form of MR in mouse serum, we studied the sites of synthesis of MR mRNA and expression of MR protein in normal mouse tissues. We demonstrate that synthesis and expression occur at identical sites, and that mature Mφ and endothelium are heterogeneous with respect to MR expression, additionally describing MR on perivascular microglia and glomerular mesangial cells. However, MR was not detected on DCs in situ, or on marginal zone or subcapsular sinus Mφ, both of which have MR-like binding activities. We also compared expression of MR to the binding of a recombinant probe containing the cysteine-rich domain of MR. We show that MR and its putative ligand(s) are expressed at nonoverlapping sites within lymphoid organs, consistent with a transfer function for soluble MR. Therefore, in addition to endocytic and phagocytic roles, MR may play an important role in antigen recognition and transport within lymphoid organs. mannose receptor macrophage dendritic cell endothelium mesangial cell Footnotes Abbreviations used in this paper: CR cysteine-rich domain of MR DC dendritic cell ICC immunocytochemistry ISH in situ hybridization MHCII major histocompatibility complex class II Mφ macrophage(s) MR mannose receptor Sn sialoadhesin sMR soluble MR Submitted: 26 March 1999 TI - Mannose Receptor and Its Putative Ligands in Normal Murine Lymphoid and Nonlymphoid Organs: In Situ Expression of Mannose Receptor by Selected Macrophages, Endothelial Cells, Perivascular Microglia, and Mesangial Cells, but not Dendritic Cells JF - The Journal of Experimental Medicine DO - 10.1084/jem.189.12.1961 DA - 1999-06-21 UR - https://www.deepdyve.com/lp/rockefeller-university-press/mannose-receptor-and-its-putative-ligands-in-normal-murine-lymphoid-8dVmZ4QuSp SP - 1961 VL - 189 IS - 12 DP - DeepDyve ER -