TY - JOUR
AU1 - Azar, Dimitri T.
AU2 - Spurr-Michaud, Sandra J.
AU3 - Tisdale, Ann S.
AU4 - Moore, Mary Beth
AU5 - Gipson, Ilene K.
AB - Abstract • Ten epikeratoplasty lenticules removed after surgery were obtained for immunohistochemical and electron microscopic analysis to determine the pattern of re-formation of corneal epithelial adhesion structures. Antibodies to laminin and type VII collagen were used to determine the presence of basement membrane and anchoring fibrils, respectively. Electron micrographs were used to determine the percentage of basal cell membrane occupied by hemidesmosomes, the area of basal lamina per 100 μg of basal cell membrane, and the average maximum depth of penetration of anchoring fibrils into the stroma. Nine normal corneas served as controls. Compared with normal corneas (24.5% of basal cell membrane occupied by hemidesmosomes; 32.0 μm2 basal lamina per 100 μm of basal cell membrane), lenticules removed for optical reasons had near-normal hemidemosomes as early as 10 weeks following surgery (mean, 20.3%). The area of basement membrane was reduced (16 μm2 basal lamina per 100 μm of basement cell membrane). During the course of 2 to 3 years, irregularities and duplications of the basement membrane were noted. Compared with normal corneas, the two lenticules removed for persistent defects had a marked reduction of hemidesmosomes and basement membrane present under epithelium at 3 and 4 weeks (9.6% of basal cell membrane occupied by hemidesmosomes and 13.6 μm2 basal lamina per 100 μm of basal cell membrane, and 5.4% of basal cell membrane occupied by hemidesmosomes and 7.2 μm2 basal lamina per 100 μm of basal cell membrane, respectively). References 1. Gipson IK, Grill SM, Spurr SJ, Brennan SJ. Hemidesmosome formation in vitro . J Cell Biol . 1983;97:849-857.Crossref 2. Westgate GE, Weaver AC, Couchman JR. Bullous pemphigoid antigen localization suggests an intracellular association with hemidesmosomes . J Invest Dermatol . 1985;84:218-224.Crossref 3. 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Binder PS, Zavala EY. Why do some epikeratoplasties fail? Arch Ophthalmol . 1987;105:63-69.Crossref 22. Lass JH, Stocker EG, Fritz ME, Collie DM. Epikeratoplasty: the surgical correction of aphakia, myopia, and keratoconus . Ophthalmology . 1987;94:912-925.Crossref 23. Yamaguchi T, Koenig SB, Kimura T, Werblin TP, McDonald MB, Kaufman HE. Histological study of epikeratophakia in primates . Ophthalmic Surg . 1984;15:230-235. 24. Pokorny KS, Kenyon KR, Swinger C, et al. Histopathology of human keratorefractive lenticules . Cornea . 1990;9:223-233.Crossref 25. Tamaki K, Yamaguchi T, McDonald MB, Kaufman HE. Histological study of epikeratophakia tissue lenses for myopia removed from two patients . Ophthalmology . 1986;93:1502-1508.Crossref 26. Arffa RC, Ebato B. Attachment of cultured corneal epithelial sheets to epikeratophakia lenticules . CLAO J . 1989;15:74-77.
TI - Reassembly of the Corneal Epithelial Adhesion Structures Following Human Epikeratoplasty
JF - Archives of Ophthalmology
DO - 10.1001/archopht.1991.01080090105032
DA - 1991-09-01
UR - https://www.deepdyve.com/lp/american-medical-association/reassembly-of-the-corneal-epithelial-adhesion-structures-following-0g0tA2ZtU0
SP - 1279
EP - 1284
VL - 109
IS - 9
DP - DeepDyve