TY - JOUR
AU - Sakiyama,, Fumio
AB - Abstract Subunit d of H+-ATP synthase from rat liver mitochondria was isolated from the purified enzyme by reverse-phase high performance liquid chromatography. The partial amino acid sequence of the subunit was determined by automated Edman degradation of the peptide fragments. The nucleotide sequence of subunit d of rat liver H+-ATP synthase was determined from a recombinant cDNA clone isolated by screening a rat hepatoma cell line H4TG cDNA library with a probe DNA. The sequence was composed of 581 nucleotides including a coding region for the import precursor of subunit d and noncoding regions on the 5- and 3' -sides. The possible precursor of subunit d and its mature polypeptide deduced from the open reading frame consisted of 161 and.160 amino acid residues with molecular weights of 18, 763 and 18, 631, respectively. Subunit d is a hydrophilic protein with an isoelectric point of 6.19. The sequence of the rat subunit d is highly homologous with that of subunit d of bovine heart and slightly similar to that of the subunit d of the yeast mitochondria. However, it had no homology with the sequence of any of the subunits of bacterial or chloroplast H+-ATP synthase. This content is only available as a PDF. Author notes 1The nucleotide sequence data reported in this paper will appear in the DDBJ, EMBL, and GenBank nucleotide sequence databases with the accession number D13120. This work was supported by Grants-in-Aid for Scientific Research [No. 05680555 (to T.H.)] and for Scientific Research on Priority Areas of Cell Energy [No. 05259219 (to T.H.)] and New Functionality Materials [No. 02204009 (to Prof. Masuo Aizawa, Tokyo Institute of Technology) ] from the Ministry of Education, Science and Culture of Japan. 3Present address: Nagahama Institute for Biochemical Science, Oriental Yeast Co., Ltd., Nagahama, Shiga 526. © 1993 BY THE JOURNAL OF BIOCHEMISTRY
TI - The Complete Amino Acid Sequence of Subunit d of Rat Liver Mitochondrial H+-ATP Synthase
JO - The Journal of Biochemistry
DO - 10.1093/oxfordjournals.jbchem.a124242
DA - 1993-11-01
UR - https://www.deepdyve.com/lp/oxford-university-press/the-complete-amino-acid-sequence-of-subunit-d-of-rat-liver-zOfJkKYz2l
SP - 714
EP - 717
VL - 114
IS - 5
DP - DeepDyve
ER -