TY - JOUR
AU - Noble, Lizanne
AB - Though guanosine triphosphate (GTP) regulatory proteins are crucial components in signal transduction by most soluble and opsonized particulate stimuli, previous data suggest that neutrophil (PMNL) activation by unopsonized hyphae differs. Most ofthe PMNL superoxide response evoked by unopsonized hyphae was independent of both Ca++ ions and pertussis toxin-sensitive guanine nucleotide regulatory proteins. To determine whether related regulatory proteins were involved in PMNL activation by unopsonized hyphae, separated PMNL plasma membranes were incubated with GTP and a poorly hydrolyzed, radiolabeled GTP analogue, 5′-guanylylimidodiphosphate, then stimulated. Particulate Candida albicans hyphae and soluble chemotacticpeptide induced comparable guanine nucleotide release. In contrast, while unopsonized hyphae caused release, it was considerably delayed, though opsonization discernibly affected neither PMNL attachment nor spreading over hyphal surfaces. This paralleled earlier observations of other delayed responses by intact PMNL to unopsonized hyphae: phospholipase C activation, the rise in cytosolic free Ca++ ions, and actin polymerization.
TI - Patterns of Guanine Nucleotide Exchange Reflecting Disparate Neutrophil Activation Pathways by Opsonized and Unopsonized Candida albicans Hyphae
JF - The Journal of Infectious Diseases
DO - 10.1093/infdis/162.1.262
DA - 1990-07-01
UR - https://www.deepdyve.com/lp/oxford-university-press/patterns-of-guanine-nucleotide-exchange-reflecting-disparate-yfv63fGLwb
SP - 262
EP - 265
VL - 162
IS - 1
DP - DeepDyve
ER -