TY - JOUR
AU - Hall, Adam R.
AB - While conventional solid-state nanopore measurements utilize ionic current, there is a growing interest in alternative sensing paradigms, including optical detection. However, a limiting factor in the application of optical schemes in particular is the inherent background fluorescence created by the solid-state membrane itself, which can interfere with the desired signal and place restrictions on the fluorophores that can be employed. An ideal device would incorporate a localized reduction in membrane fluorescence using a method that can be integrated easily with the nanopore fabrication process. Here, we demonstrate that in addition to forming nanopores and nanopore arrays, a focused helium ion beam can be used to reduce the fluorescence of a conventional silicon nitride membrane controllably. The reduction in background produces low-fluorescence devices that can be used for optical detection of double-strand DNA, as well as for conventional resistive pulse sensing. This approach is used to identify the translocation of short single-strand DNA through individual nanopores within an array, creating potential for a massively-parallel detection scheme.
TI - Solid-state nanopores and nanopore arrays optimized for optical detection
JF - Nanoscale
DO - 10.1039/c4nr00305e
DA - 2014-05-29
UR - https://www.deepdyve.com/lp/royal-society-of-chemistry/solid-state-nanopores-and-nanopore-arrays-optimized-for-optical-yFxQfu0N0p
SP - 6991
EP - 6996
VL - 6
IS - 12
DP - DeepDyve
ER -