TY - JOUR
AU1 - Wang, Yue
AU2 - Wang, Hui-Li
AU3 - Lin, Zhi-Ping
AU4 - Zhong, Ji-Feng
AU5 - Chen, Kun-Lin
AU6 - Duan, Xing
AB - BackgroundExosomes are involved in intercellular communication, affecting many physiological and pathological process. The present study evaluated the effects of serum exosomes on the function of bovine mammary epithelial cells (BMECs) and milk synthesis under heat stress.Methods and resultsWe cultured the BMECs in fetal bovine serum (FBS) or exosome-free FBS medium and examined, their viability using CCK-8 kit. The results showed that culturing the cells in an exosome-free medium decreased viability and increased the levels of reactive oxygen species. The BMECs cultured in the exosome-free medium had reduced mitochondrial membrane potential, decreased manganese superoxide dismutase activity, and disrupted mitochondrial dynamics. They exhibited apoptosis due to upregulated Drp1, Fis1, Bax and HSP70. Lastly, we observed downregulation of milk fat and lactoprotein-related genes: mTOR, PPARγ, p-mTOR and ADD1 and SREBP1, ELF5, and CSN2, respectively, after culturing the cells in an exosome-free medium. These negative effects of the exosome-free medium on the BMECs could be further reinforced under heat stress.ConclusionOur results demonstrated that exosomes from serum are critical for maintaining the normal function of BMECs.
TI - Depletion of serum-derived exosomes aggravates heat stress-induced damage of bovine mammary epithelial cells
JF - Molecular Biology Reports
DO - 10.1007/s11033-022-07767-6
DA - 2022-10-01
UR - https://www.deepdyve.com/lp/springer-journals/depletion-of-serum-derived-exosomes-aggravates-heat-stress-induced-y7c0kNU8hD
SP - 9297
EP - 9305
VL - 49
IS - 10
DP - DeepDyve
ER -