TY - JOUR
AB - Reviews Correlation of organelle dynamics between light microscopic live imaging and electron microscopic 3D architecture using FIB-SEM Keisuke Ohta, Shingo Hirashima, Yoshihiro Miyazono, Akinobu Togo and Kei-ichiro Nakamura Correlative light and electron microscopy (CLEM) methods combined with live imaging can be applied to understand the dynamics of organelles. However, many researchers have difficulties in applying this technique due to technical issues. Here, we propose a practical live-CLEM method combined with FIB-SEM, which reduce such technical barriers. Microscopy 2021 70:2 161--170; doi:10.1093/jmicro/dfaa071 Local structure analysis of amorphous materials by angstrom-beam electron diffraction Akihiko Hirata The angstrom-beam electron diffraction method was used to obtain the local structure information of amorphous materials at a sub-nanometre scale. Some applications of the proposed method to structural and functional amorphous materials are summarised. Microscopy 2021 70:2 171--177; doi:10.1093/jmicro/dfaa075 Articles Evaluation of accuracy in the determination of crystal structure factors using large-angle convergent-beam electron diffraction patterns Daisuke Morikawa and Kenji Tsuda Accuracy of electron density distribution analysis using large-angle convergent-beam electron diffraction (LACBED) patterns was evaluated. Electron density distribution of the Fe 3d orbitals were successfully reconstructed from LACBED patterns with convergence angles larger than 15.2 mrad, which is 4 times as large as that for the conventional CBED patterns. Microscopy 2021 70:2 178--185; doi:10.1093/jmicro/dfaa041 Visualization of microstructural change affected by mechanical stimulation in tendon healingwith a novel tensionless model Junya Oshima, Kaoru Sasaki, Naoto Yamamoto, Tomoharu Kiyosawa and Mitsuru Sekido We created the tensionless tendon laceration model by transplanting the leg of a rat to the back, removing mechanical stimulation. Under tension, collagen at the tendon stump shows axial alignment and repair that subsequently demarcates the paratenon border. In contrast, under tensionless conditions, the collagen remains randomly arranged. Microscopy 2021 70:2 186--191; doi:10.1093/jmicro/dfaa043 Three-dimensional culture of a pancreatic cancer cell line, SUIT-58, with air exposure can reflect the intrinsic features of the original tumor through electron microscopy Nobuyasu Takahashi, Fumiyo Aoyama and Akira Sawaguchi Application of a three-dimensional culture system with air exposure facilitates the formation of large cell spheres possessing cribriform glands and producing mucin in the collagen gel. Transmission electron microscopy revealed the formation of microvilli and junctional complexes at the apical side of the cell. Microscopy 2021 70:2 192--200; doi:10.1093/jmicro/dfaa046 Histochemical assessment on the cellular interplay of vascular endothelial cells and septoclasts during endochondral ossification in mice Erika Tsuchiya, Tomoka Hasegawa, Hiromi Hongo, Tomomaya Yamamoto, Miki Abe, Taiji Yoshida, Shen Zhao, Kanako Tsuboi, Nobuyuki Udagawa, Paulo Henrique Luiz de Freitas, Minqi Li, Yoshimasa Kitagawa and Norio Amizuka Our original article demonstrated that vascular endothelial cells adjacent to the chondro-osseous junction would interplay with septoclasts for synchronous invasion into the epiphyseal cartilage, while those slightly distant from the chondro-osseous junction would cooperate with osteoblastic activities presumably by mediating EphB4/ephrinB2. Microscopy 2021 70:2 201--214; doi:10.1093/jmicro/dfaa047 Sandwich freezing device for rapid freezing of viruses, bacteria, yeast, cultured cells and animal and human tissues in electron microscopy Masashi Yamaguchi, Masaki Taguchi, Katsuyuki Uematsu, Azusa Takahashi-Nakaguchi, Michiyo Sato-Okamoto and Hiroji Chibana We fabricated and commercialized a new sandwich freezing device. The new device is inexpensive, portable, and sterilizable. It can be used to rapid-freeze viruses, bacteria, yeast, cultured cells, and animal and human tissues to a depth of 0.2 mm if tissues are prefixed with glutaraldehyde. Microscopy 2021 70:2 215--223; doi:10.1093/jmicro/dfaa049 Three-dimensional structure analysis of melanocytes and keratinocytes in senile lentigo Yuki Mizutani, Mika Yamashita, Rie Hashimoto, Toru Atsugi, Akemi Ryu, Akinobu Hayashi, Yukiko Rikimaru-Nishi and Keisuke Ohta By using several microscopic methods, we have elucidated the three-dimensional morphology of melanocytes and keratinocytes in senile lentigo skin. Focused ion beam-scanning electron micrographs showed that dendritic processes in senile lentigo skin encircled the adjacent keratinocytes and accumulated a large number of melanosomes that contribute to efficient melanosome transfer. Microscopy 2021 70:2 224--231; doi:10.1093/jmicro/dfaa054 Advances in cryo-EM and ED with a cold-field emission beam and energy filtration ---Refinements of the CRYO ARM 300 system in RIKEN SPring-8 center--- Saori Maki-Yonekura, Tasuku Hamaguchi, Hisashi Naitow, Kiyofumi Takaba and Koji Yonekura We report on the characteristics of a cryo-EM system with a cold-field emission gun and an energy filter designed for higher-resolution single particle analysis and high-precision electron 3D crystallography. The progress and updates in the past ∼ 22 months are also presented including high quality data in both the applications. Microscopy 2021 70:2 232--240; doi:10.1093/jmicro/dfaa052 Improving the depth resolution of STEM-ADF sectioning by 3D deconvolution A. Ishizuka, K. Ishizuka, R. Ishikawa, N. Shibata, Y. Ikuhara, H. Hashiguchi and R. Sagawa The 3D deconvolution routines applicable to the STEM though-focus ADF images were developed to improve the depth resolution. The depth locations can be determined within 0.1–0.2 nm, since the deconvolution gives a smooth scattering distribution with a high-P/B ratio. The new routines work with a limited defocus-range of STEM-ADF images. Microscopy 2021 70:2 241--249; doi:10.1093/jmicro/dfaa056 Technical Report Novel –|$\boldsymbol{75}^{\circ}$|C SEM cooling stage: application for martensitic transformation in steel Kaneaki Tsuzazki, Motomichi Koyama, Ryosuke Sasaki, Keiichiro Nakafuji, Kazushi Oie, Akinobu Shibata, Takashi Gondo, Hiroya Miyazaki, Hiroshi Akamine and Minoru Nishida A SEM cooling stage available to −75 °C has been developed and applied for observation of martensitic transformation in Fe-31 mass% Ni alloy. We introduced applicability of SEM-ECCI analysis, which is available for dislocation-resolved in situ characterization. Two frame-by-frame movies are available in the supplemental data. Microscopy 2021 70:2 250--254; doi:10.1093/jmicro/dfaa051 © The Author(s) 2020. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
TI - In This Issue
JF - Microscopy
DO - 10.1093/jmicro/dfab010
DA - 2021-03-24
UR - https://www.deepdyve.com/lp/oxford-university-press/in-this-issue-xhU4IrLRj0
SP - 1
EP - 4
VL - 70
IS - 2
DP - DeepDyve
ER -