TY - JOUR
AU - TAKEDA,, JUNKO
AB - Abstract Various agents were tested for the effects on both the electrofusion and the subsequent rounding up (spherulation) of fused protoplasts from barley (Hordeum vulgare var. Moor). The microfilament (MF) inhibitor, cytochalasin D (CD), had no effect on the frequency of fusion, but greatly increased the frequency of spherulation. The effects of CD were rapid, long-lived and maximal at 2 to 10 mmol m−3. Ca2+ also promoted spherulation of fusion products, whereas phalloidin and the calcium inonophore A23187 completely abolished the effect of both CD and Ca2+. An internal Ca2+ antagonist, 8-(diethylamino)octyl 3, 4, 5-trimethoxybenzoate (TMB8) at 50 mmol m−3 also inhibited spherulation without affecting the frequency of fusion and CD could almost completely reverse its effects. The effects of CD persisted for up to 1 h after its removal, whereas the effects of Ca2+ and TMB8 were exerted at the beginning of incubation and were immediately abolished by washing. These results indicate that the effect of Ca2+ on the formation of spherical fusion products is closely correlated with the status of the microfilaments and that the internal Ca2+ concentration and, perhaps, its transient change which affects the MF, are intimately involved in the process. Cytoskeleton, electrofusion, calcium effect This content is only available as a PDF. © Oxford University Press
TI - Promotion by Calcium Ions and Cytochalasin D of the Rounding Up Process (Spherulation) of Electrofused Barley Protoplasts and its Relation to the Cytoskeleton
JF - Journal of Experimental Botany
DO - 10.1093/jxb/40.7.819
DA - 1989-07-01
UR - https://www.deepdyve.com/lp/oxford-university-press/promotion-by-calcium-ions-and-cytochalasin-d-of-the-rounding-up-xLoJBJei0h
SP - 819
EP - 826
VL - 40
IS - 7
DP - DeepDyve
ER -