TY - JOUR
AU - Pleasure,, David
AB - Abstract Oligodendrocytes isolated from the corpus callosum of four-week-old rats by trypsinization and Percoll density gradient centrifugation were cultured on poly- 1-lysine coated coverslips. Some cells extended short processes within 24 hours (h), and at that time up to 95% of the cells showed surface binding of rabbit antiserum to galactocerebroside (anti-GalC) as demonstrated by indirect immunofluorescence. Oligodendrocytes survived up to two months in culture, extending processes with membranous elaborations. Exposure of living oligodendrocytes to varying dilutions of rabbit anti-GalC serum in the presence of complement produced cytotoxicity which was directly proportional to the concentration of antiserum and duration of exposure, as assessed by a nigrosin dye exclusion test. This system of isolating and culturing rat oligodendrocytes will permit further developmental and immunologic studies related to demyelinating diseases. Cytotoxicity test, immunologic, Galactocerebrosides, Oligodendrocytes, Tissue culture This content is only available as a PDF. Author notes Supported by NIH Grant NS-11037 and the National Multiple Sclerosis Society. Copyright © 1983 by the American Association of Neuropathologists
TI - Long-term Culture of Oligodendrocytes Isolated from Rat Corpus Callosum by Percoll Density GradientLysis by Polyclonal Antigalactocerebroside Serum
JO - Journal of Neuropathology & Experimental Neurology
DO - 10.1097/00005072-198301000-00002
DA - 1983-01-01
UR - https://www.deepdyve.com/lp/oxford-university-press/long-term-culture-of-oligodendrocytes-isolated-from-rat-corpus-xDHlre8Mo6
SP - 16
EP - 28
VL - 42
IS - 1
DP - DeepDyve
ER -