TY - JOUR
AU - Siracusano, A
AB - SUMMARY The role of cytokines in human hydatidosJs (Echinococcus granulosus infection) was evaluated in immunoassays determining production of IL-4, IL-10 and interferon-gamma (IFN-γ) in peripheral blood mononuclear cell (PBMC) cultures from 30 hydatid patients and 14 uninfected controls. In ceil cultures from hydatid patients parasite and non-parasite antigen stimulation significantly increased IL-4 production (P·0·005). Spontaneous and milogen-driven IL-4 production was similar in patients and controls. IL-10 and IFN-γ production did not differ statistically in the two groups, even though some hydatid patients produced these cytokines in large amounts. Notably, antigen-driven IFN-γ concentrations were invariably higher in patients than in uninfected controls. Data analysis showed a relationship between IgE and IgG4 responses and parasite-driven cytokine production. High IgE and IgG4 responders produced high IL-4 and IL-10 concentrations. High IgE responders showed decreased IFN-γ production, but high IgG4 responders had IFN-γ levels slightly higher than those of low responders. Cytokine response patterns did not relate to the clinical stage of disease. The significantly increased IL-4 and the high IL-10 concentrations found in PBMC from many hydatid patients in this study are consistent with Th2 cell activation in human hydatidosis. The presence of antigen-driven IFN-γ production in patients with E. granulosus infection implies concurrent intervention of the Th1 or Th0 cell subset. Echinococcus granulosus, cytokines Th1 and Th2 cells, IgB/IgG4 References 1 Afferni C , Pini C, Misiti-Dorello P et al. Detection of specific IgE antibodies in sera from patients with hydatidosis . Clin Exp Immunol 1984 ; 55 : 587 – 92 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 2 Aceti A , Pennica A, Teggi A et al. IgG subclasses in human hydatid disease: prominence of the IgG4 response . Int Arch Allergy Immunol 1993 ; 102 : 347 – 51 . Google Scholar Crossref Search ADS PubMed WorldCat 3 Williams JF. Recent advances in the immunology of cestode infections . J Parasitol 1979 ; 65 : 337 – 49 . Google Scholar Crossref Search ADS PubMed WorldCat 4 Mosmann TR , Cherwinski H, Bond MW et al. Two types of murine helper T cell clones: I. Definition according to profiles of lymphokine activities and secreted proteins . J Immunol 1986 ; 136 : 2348 – 57 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 5 Romagnani S. Human Th1 and Th2 subsets: doubt no more . Immunol Today 1991 ; 12 : 256 – 7 . Google Scholar Crossref Search ADS PubMed WorldCat 6 Romagnani S. Human Th1 and Th2 subsets: regulation of differentiation and role in protection and immunopathology . Int Arch Allergy Appt Immunol 1992 ; 98 : 279 – 85 . Google Scholar Crossref Search ADS WorldCat 7 King CL. Nutman TB. IgE and IgG subclass regulation by IL-4 and IFN-T in human helminth infections. Assessment by B cell precursor frequencies . J Immunol 1993 ; 151 : 58 – 65 . Google Scholar OpenURL Placeholder Text WorldCat 8 Ishizaka A , Joh K, Shibata R et al. Regulation of IgE and IgG4 synthesis in patients with hyper IgE syndrome . Immunoiogy 1990 ; 70 : 414 – 6 . Google Scholar OpenURL Placeholder Text WorldCat 9 Clutterbuck E , Hirst E, Sanderson C. Human interleukin 5 (IL-5) regulates the production of eosinophils in human bone marrow cultures: comparison and interaction with IL-1, IL-3, IL-6, and GM-CSF . Blood 1989 ; 73 : 1504 – 12 . Google Scholar Crossref Search ADS PubMed WorldCat 10 Finkelman FD , Pearce EJ, Urban JF Jr et al. Regulation and biological function of helminth-induced cytokine responses , immunol Today 1991 ; 12 : A62 – A66 . Google Scholar Crossref Search ADS PubMed WorldCat 11 Scott P , Pearce EJ, Cheever AW et al. Role of cytokines and CD4+ T-cell subsets in the regulation of parasite immunity and disease . Immunol Rev 1989 ; 112 : 161 – 82 . Google Scholar Crossref Search ADS PubMed WorldCat 12 Grau GE , Modlin RL. Immune mechanisms in bacterial and parasitic diseases: protective immunity versus pathology . Curr Opin Immunol 1991 ; 3 : 480 – 5 . Google Scholar Crossref Search ADS PubMed WorldCat 13 Pearce EJ , Caspar P, Griych JM et al. Downregulation of Th1 cytokine production accompanies induction of Th2 responses by a parasitic helminth, Schistosoma mansoni. J Exp Med 1991 ; 173 : 159 – 66 . Google Scholar Crossref Search ADS PubMed WorldCat 14 Romagnani S. Type 1 T helper and type 2 T helper cells: functions, regulation and role in protection and disease . Int J Clin Lab Rea 1991 ; 21 : 152 – 8 . Google Scholar Crossref Search ADS WorldCat 15 Chomarat P. Rissoan M, Banchereau J et al. Interferon inhibits interleukin-10 production by monocytes . J Exp Med 1993 ; 177 : 523 – 7 . Google Scholar Crossref Search ADS PubMed WorldCat 16 Burdin N. Péronne C. Banchereau J et al. Epstein-Barr virus-transformation induces B lymphocytes to produce human inter-leukin-10 . J Exp Med 1993 ; 177 : 295 – 304 . Google Scholar Crossref Search ADS PubMed WorldCat 17 Sher A , Fiorentino D, Caspar P et al. Production of IL-10 by CD4+ T lymphocytes correlates with down-regulation of Th1 cytokine synthesis in helminth infection . J Immunol 1991 ; 147 : 2713 – 6 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 18 Mosmann TR , Moore KW. The role of IL-10 in crossregulation of Th1 and Th2 responses . Immunol Today 1991 ; 12 : A49 – A53 . Google Scholar Crossref Search ADS PubMed WorldCat 19 Siracusano A , Teggi A, Quintieri F et al. Cellular immune responses of hydatid patients to Echinococcus granulosus antigens . Clin Exp Immunol 1988 ; 72 : 400 – 5 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 20 Profumo E , Orlona E, Riganò R et al. Cellular and humoral responses to antigenic subunits of Echinococcus granulosus cyst fluid in hydatid patients . Parasite Immunol 1994 ; 16 : 575 – 80 . Google Scholar Crossref Search ADS WorldCat 21 Bombardieri S , Giordano F, Ingrao F et al. An evaluation of an agar gel diffusion test with crude and purified antigens in the diagnosis of hydatid disease . Bull WHO 1974 ; 51 : 525 – 30 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 22 Oriol R , Williams JF, Perez-Esandi MV et al. Evaluation of purified lipoprotein antigens of Echinococcus granutosus in the immunodiagnosis of human infection . Am J Trop Med Hyg 1971 ; 20 : 569 – 74 . Google Scholar Crossref Search ADS PubMed WorldCat 23 Bradford MM. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding . Anal Biochem 1976 ; 72 : 248 – 54 . Google Scholar Crossref Search ADS PubMed WorldCat 24 Challacombe SJ , Biggerstaff M, Greenall C, et al. ELISA detection of IgG subclass antibodies to Streptococcus mutans. J Immunol Methods 1986 ; 87 : 95 – 102 . Google Scholar Crossref Search ADS PubMed WorldCat 25 Ricci M , Rossi O. Dysregulation of IgE responses and airway allergic inflammation in atopic individuals . Clin Exp All 1990 ; 20 : 601 – 9 . Google Scholar Crossref Search ADS WorldCat 26 Bonnefoy JY , Pochon S, Aubry JP et al. A new pair of surface molecules involved in human IgE regulation . Immunol Today 1993 ; 14 : 1 – 2 . Google Scholar Crossref Search ADS PubMed WorldCat 27 Bezzi A , Teggi A, de Rosa F et al. Abdominal hydatid disease: U.S. findings during medical treatment . Radiology 1987 ; 162 : 91 – 95 . Google Scholar Crossref Search ADS PubMed WorldCat 28 Teggi A , Lastilla MG, de Rosa F. Therapy of human hydatid disease with Mebendazole and Albendazole . Antimicrob Agents Chemoler 1993 ; 37 : 1679 – 84 . Google Scholar Crossref Search ADS WorldCat This content is only available as a PDF. © 1995 Blackwell Science Ltd This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
TI - In vitro production of cytokines by peripheral blood mononuclear cells from hydatid patients
JO - Clinical & Experimental Immunology
DO - 10.1111/j.1365-2249.1995.tb05569.x
DA - 2008-06-28
UR - https://www.deepdyve.com/lp/oxford-university-press/in-vitro-production-of-cytokines-by-peripheral-blood-mononuclear-cells-x8pbSPUnRo
SP - 433
EP - 439
VL - 99
IS - 3
DP - DeepDyve
ER -