TY - JOUR
AU - Grinyó, J M
AB - SUMMARY Clonal deletion or inactivation of donor-specific alloreactive cells are important mechanisms that are believed to account for acquired immune tolerance in allograft recipients. Serial assessment of precursor cytotoxic T lymphocyte frequencies (CTLpf) by limiting dilution analysis (LDA) provides information at the clonal level on changes in the alloimmune response of graft recipients. We performed a longitudinal study of 15 cadaveric kidney recipients before and every 3 months throughout the first year after transplantation (Tx). Pre-Tx values of donor CTLpf showed high interindividual variability without a predictive value for the clinical outcome. All patients with well functioning kidneys had decreased CTLpf at 3 months post-Tx in comparison with pre-Tx values. This decrease was donor-specific in four patients and was permanent in two cases throughout the study. Most patients presented decreased anti-donor CTLpf values from 6 to 9 months, whereas a partial recovery of donor CTLpf was observed in three patients. Reversible acute rejection was diagnosed in three patients, and it was associated with a marked increase in anti-donor CTLpf, returning to pre-Tx values by 9 months post-Tx. In addition, one patient with chronic rejection displayed a transient increase in CTLpf 6 months after Tx. The results of this sequential study indicate the establishment of a state of either hyporesponsiveness or functional clonal inactivation, transient or permanent, which could facilitate allograft acceptance. limiting dilution assay, kidney, transplantation, clonal deletion This content is only available as a PDF. © 1996 Blackwell Science Ltd This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
TI - Longitudinal study of the frequency of cytotoxic T cell precursors in kidney allograft recipients
JF - Clinical & Experimental Immunology
DO - 10.1046/j.1365-2249.1996.d01-657.x
DA - 2003-10-29
UR - https://www.deepdyve.com/lp/oxford-university-press/longitudinal-study-of-the-frequency-of-cytotoxic-t-cell-precursors-in-wxbCVf7aAg
SP - 108
EP - 114
VL - 104
IS - 1
DP - DeepDyve
ER -