TY - JOUR
AU - Zinovieva, N. A.
AB - Abstract Availability of high-density SNP arrays created an opportunity to understand current genetic structure and differentiation of sheep breeds and to find ways for their improving via whole genome analysis. A wide range of sheep breeds including wool, meat, milk types, and dual- and multi-purpose breeds was established in Russia. Although the breeds have unique gene pool and are a part of national heritage, they have not been genotyped yet and there is no information of their polymorphism at genome level. In this regard, our aim was to evaluate genetic diversity and population structure of some Russian sheep breeds. We used OvineSNP50K BeadChip to genotype 141 sheep of 11 breeds including Romanov (ROM, n = 22), Baikal fine-fleeced (ZBL, n = 12), Tuvan short fat tailed (TUV, n = 16), Kuibyshev (KUI, n = 11), Soviet Merino (SVM, n = 10), Kuchugur (KCH, n = 12), Karakul (KAR, n = 16), North Caucasian merino (NCM, n = 11), Russian long haired (RLH, n = 11), Stavropol (STA, n = 10), and Manych merino (MAN, n = 10). Quality control (QC) of SNPs and summary statistics were performed in PLINK v1.07. R v3.2.3 was used to create input files and visualize the data. After QC overall 48,842 SNPs (90%) were involved in the further analysis, 47,980 (or 98.3% from detected) were polymorphic. The highest polymorphism level among the breeds was identified in ZBL (97.4%), whereas KCH, RLH, STA were characterized by 93.4, 93.7, and 93.8% of polymorphic loci, respectively. Observed heterozygosity was 0.370 ranging from 0.361 in ROM to 0.398 in ZBL. All breeds were characterized by heterozygote excess ranging from 0.84 in ROM to 9.78% in KCH. The minor allele frequency was 0.305 overall for all breeds. The MDS analysis showed pattern corresponding breeds productivity type. Merino breeds (STA, SVM, MAN, ZBL) with close NCM group and semi-fine fleeced clade of RLH and KUI clustered separately from KAR and TUV with coarse wool. Multi-purpose KCH occupied an equidistant position. The most remote group was ROM, probably, due to their unique genetic traits such as extremely high prolificacy and adaptability to any conditions of keeping and feeding. Our study represents the initial phase of large-scale SNP genotyping of Russian sheep breeds. The research was conducted under financial support of Russian Scientific Foundation (project N° 14-36-00039). PDF This content is only available as a PDF. American Society of Animal Science © American Society of Animal Science 2016
TI - 1711 Genomic evaluation and population structure of eleven Russian sheep breeds
JO - Journal of Animal Science
DO - 10.2527/jam2016-1711
DA - 2016-10-01
UR - https://www.deepdyve.com/lp/oxford-university-press/1711-genomic-evaluation-and-population-structure-of-eleven-russian-wlSua32NFt
SP - 834
EP - 834
VL - 94
IS - suppl_5
DP - DeepDyve
ER -