TY - JOUR
AU - Stock, C., Chester
AB - Abstract The possible relationship between ganglioside levels and ganglioside profiles in malignant tumors and the formation of metastasis was investigated by the analysis of gangliosides in metastasizing SMT-2A and nonmetastasizing MT-W9a mammary carcinomas as well as in metastases formed from SMT-2A tumors. The extracted lipid of SMT-2A tumors contained 3.3-fold more lipid-bound sialic acid than did that of MT-W9a tumors. The differences were also substantial in the ganglioside profiles in these 2 tumors. Plasma membranes isolated from SMT-2A tumors also contained 1.8-fold more lipid-bound sialic acid than did plasma membranes from MT-W9a tumors. Ganglioside profiles in two types of SMT-2A secondary tumors were investigated. The lipid-bound sialic acid content was 1.5-fold higher in tumor nodules in the lung and 1.9-fold higher in axillary lymph node tumors than it was in primary SMT-2A tumors. The ganglioside pattern in these 2 secondary tumors generally reflected that found in SMT-2A: high levels of gangliosides containing three or four sialic acid molecules. The lung nodule retained its specificity with respect to lipid-bound sialic acid content and ganglioside pattern after the lung nodule was sequentially transplanted three times to the site of the original SMT-2A tumor growth. 2 Supported by Public Health Service grant 5-R01-CA24419 and by core grant 08748 from the National Cancer Institute; by a grant from the Elsa U. Pardee Foundation; and by Sloan-Kettering Institute for Cancer Research biomedical research support grant S07-RR05534 from the Division of Research Services, National Institutes of Health. 3 Nomenclature used for gangliosides is according to Svennerholm (1). 4 A preliminary account (abstract No. 79) of this work was presented at the annual meeting of the Society for Complex Carbohydrates, Boston, Mass., Sept. 25–27, 1980. This content is only available as a PDF. Author notes 5 Memorial Sloan-Kettering Cancer Center, 1275 York Ave., New York, N.Y. 10021. 7 We thank Dr. Lars Svennerholm (Psychiatric Research Center, St. Jorgen Hospital, Göteborg, Sweden), Dr. Robert K. Yu (Yale University, New Haven, Conn.), and Dr. David A. Wenger (University of Colorado Medical Center, Denver, Colo.) for their valuable gifts of commercially unavailable ganglioside species. We also thank Dr. Arthur E. Bogden and Dr. Joseph Gosselin (Tumor Bank, Mason Research Institute, Worcester, Mass.) for the SMT-2A and MT-W9a mammary carcinomas and thank Dr. Maria de Sousa (Memorial Sloan-Kettering Cancer Center, New York, N.Y.) for consultation.
TI - Ganglioside Profiles of Metastases and of Metastasizing and Nonmetastasizing Rat Primary Mammary Carcinomas
JF - JNCI: Journal of the National Cancer Institute
DO - 10.1093/jnci/67.6.1251
DA - 1981-12-01
UR - https://www.deepdyve.com/lp/oxford-university-press/ganglioside-profiles-of-metastases-and-of-metastasizing-and-wic0Lk9Qwc
SP - 1251
EP - 1258
VL - 67
IS - 6
DP - DeepDyve
ER -