TY - JOUR
AU - Cen, Pei-Lin
AB - A simple, reliable and low-cost agar diffusion bioassay for quantitative determination of mildiomycin was developed using a strain of Rhodotorula rubra AS 2.166 as the indicator organism and potato dextrose agar at pH 7.0 as the test medium. With equivalent precision and accuracy to HPLC analysis, this method was applied to analyse mildiomycin in complex culture broth during the fermentation process. A modified agar plug method based on the bioassay was constructed for rapid and efficient screening of high-yielding mutants of mildiomycin. Within four weeks, a high production strain, the mildiomycin productivity of which was 75.5% higher than the parent strain, was obtained from 15,000 mutants.
TI - Bioassay of Mildiomycin and a Rapid, Cost-Effective Agar Plug Method for Screening High-yielding Mutants of Mildiomycin
JF - World Journal of Microbiology and Biotechnology
DO - 10.1007/s11274-005-6561-6
DA - 2005-04-27
UR - https://www.deepdyve.com/lp/springer-journals/bioassay-of-mildiomycin-and-a-rapid-cost-effective-agar-plug-method-wT4YBeNTt3
SP - 1433
EP - 1437
VL - 21
IS - 9
DP - DeepDyve
ER -