TY - JOUR
AU1 - Jung,, Thomas
AU2 - Fischer,, Bernd
AU3 - Beier, Henning, M.
AB - Abstract Day 4 rabbit blastocysts were cultured in Ham's F-10 medium supplemented either with homologous serum or uterine flushings. Development was assessed by leucine and methionine incorporation at 24 h and 48 h, respectively, after initiation of culture and compared with day 4 and day 5 non-cultured controls. After 24 h hi culture, incorporation data were similar for both media groups. After 48 h, a significantly higher protein synthesis activity was found in blastocysts which had access to uterine secretions in vitro. However, incorporation levels of day 5 controls were not reached. Modes of incorporation of leucine and methionine revealed to be very similar in all experimental groups but ranged on a different level. Leucine was incorporated on a 5.5-fold higher level than methionine. We conclude that (i) supplementation of culture media with uterine secretions is beneficial for blastocyst development, and (ii) further studies should focus on non-serum components within the uterine fluid and their significance for embryonic development. rabbit blastocysts, protein synthesis, uterine secretion, amino acid incorporation, in-vitro culture This content is only available as a PDF. © IRL Press Limited
TI - Quantitative aspects of protein synthesis in non-cultured and cultured rabbit blastocysts
JF - Human Reproduction
DO - 10.1093/oxfordjournals.humrep.a136483
DA - 1987-01-01
UR - https://www.deepdyve.com/lp/oxford-university-press/quantitative-aspects-of-protein-synthesis-in-non-cultured-and-cultured-w0IGedd3rV
SP - 23
EP - 27
VL - 2
IS - 1
DP - DeepDyve
ER -