TY - JOUR AU - OSHIMA,, Tairo AB - Abstract Components of a lipid-containing phage øNS11 were characterized. The phage had five protein components, the molecular weights of which were 59,000, 44,000, 33,000, 23,000, and 18,000. Viral lipid consisted of six components, which were also found in the host bacterial lipid. The relative amounts of these viral lipid components were very similar to those of the bacterial lipid. The phage contained ω-cyclohexyl fatty acids characteristic of Bacillus acidocaldarius as the main fatty acids. The phage nucleic acid was a linear double-stranded DNA, the molecular weight of which was 9.3–9.4 x 106 daltons. The guanine plus cytosine content of the DNA was determined to be about 52% from chemical analysis, buoyant density (1.711 g/cm3 in CsCl) and melting temperature (90.6°C in 0.15 M NaCl plus 0.015 M sodium citrate). The phage contained two kinds of polyamine; spermidine and spermine. This content is only available as a PDF. © COPYRIGHT, 1977 BY JOURNAL OF BIOCHEMISTRY TI - Bacteriophage øNS11: A Lipid-Containing Phage of Acidophilic Thermophilic BacteriaIII. Characterization of Viral Components JO - The Journal of Biochemistry DO - 10.1093/oxfordjournals.jbchem.a131834 DA - 1977-11-01 UR - https://www.deepdyve.com/lp/oxford-university-press/bacteriophage-ns11-a-lipid-containing-phage-of-acidophilic-ulYcRdw3OY SP - 1457 EP - 1461 VL - 82 IS - 5 DP - DeepDyve ER -