TY - JOUR
AU - Ban, R.
AB - As an intermediate in drug synthesis, uridine has practical applications in the pharmaceutical field. Bacillus subtilis is used as a host to boost uridine yield by manipulating its uridine biosynthesis pathway. In this study, we engineered a high‐uridine‐producing strain of B. subtilis by modifying its metabolic pathways in vivo. Overexpression of the aspartate ammonia‐lyase (ansB) gene increased the relative transcriptional level of ansB in B. subtilis TD320 by 13·18 times and improved uridine production to 15·13 g l−1 after 72‐h fermentation. Overexpression of the putative 6‐phosphogluconolactonase (ykgB) gene increased uridine production by the derivative strain TD325 to 15·43 g l−1. Reducing the translation of the amido phosphoribosyl transferase (purF) gene and inducing expression of the subtilisin E (aprE) gene resulted in a 1·99‐fold increase in uridine production after 24 h shaking. Finally, uridine production in the optimal strain B. subtilis TD335, which exhibited reduced urease expression, reached 17·9 g l−1 with a yield of 314 mg of uridine g−1 glucose. To our knowledge, this is the first study to obtain high‐yield uridine‐producing B. subtilis in a medium containing only three components (80 g l−1 glucose, 20 g l−1 yeast powder, and 20 g l−1 urea).
TI - Metabolic engineering of Bacillus subtilis for high‐level production of uridine from glucose
JO - Letters in Applied Microbiology
DO - 10.1111/lam.13754
DA - 2022-10-01
UR - https://www.deepdyve.com/lp/oxford-university-press/metabolic-engineering-of-bacillus-subtilis-for-high-level-production-uZ4ob9SjS8
SP - 824
EP - 830
VL - 75
IS - 4
DP - DeepDyve
ER -