TY - JOUR
AU - Pechère, Jean-Claude
AB - An unusual cephalosporinase in Achromobacter species was characterized biochemically;the enzyme had a pI of 8.1 and a molecular mass of 36,200 daltons, and it was not inhibitedby p-chloromercuribenzoate or cloxacillin. Specific antiserum neutralized enzymaticactivity. Agarose gel electrophoresis of the DNA of two strains (MULB 906 andMULB 912) revealed at least three plasmid bands; cured strains demonstrated a simultaneousloss of β-lactamase and plasmid DNA. Resistance to β-lactam antibiotics wastransferred by transformation of Escherichia coli strain HB101 with plasmid DNA. Thisplasmid-mediated β-lactamase differed from the two types of chromosomal cephalosporinases(PI 7.4 and 9.3, respectively) found in a survey of clinical isolates of Achromobacterspecies. This enzyme also differed in its biochemical properties from all of theother known plasmid-mediated β-1actamases.
TI - A Plasmid-Mediated Cepbalosporinase from Achromobacter Species
JO - The Journal of Infectious Diseases
DO - 10.1093/infdis/145.2.753
DA - 1982-05-01
UR - https://www.deepdyve.com/lp/oxford-university-press/a-plasmid-mediated-cepbalosporinase-from-achromobacter-species-uWvuvVsJyM
SP - 753
EP - 761
VL - 145
IS - 5
DP - DeepDyve
ER -