TY - JOUR
AU - Patrone,  Franco
AB - 277 51 51 2 2 Franco Dallegri Guido Frumento Alberto Ballestrero Loris Banchi Franco Patrone Second Medical Clinic University of Genova Genova Italy ISMI-Clinica Medica R Università degli Studi Viale Benedetto XV, n. 6 I-16132 Genova Italy Summary Neutrophil-derived nucleus- and granule-free cytoplasts, consisting of cytosol enclosed by an intact plasma membrane, were able to destroy 51 Cr-labelled ox red blood cells (ORBC) in the presence of phorbol myristate acetate (PMA). The slope of the target cell lysis vs the log of the cytoplast number was similar to that observed with neutrophils as effector cells. Nevertheless, a number of cytoplasts 60–80 times higher than that of neutrophils was required to obtain a common level of cytotoxicity. The ability of cytoplasts and neutrophils to lyse ORBC was completely abolished by catalase and unaffected by superoxide dismutase and mannitol, suggesting the involvement of hydrogen peroxide in the target cell damage. Addition of myeloperoxidase (MPO) to cytoplasts increased lysis. The MPO inhibitor azide significantly reduced the cytolysis by neutrophils, but not the cytolysis by cytoplasts, except when experiments were carried out in the presence of MPO. The results indicate that neutrophil cytosol and plasma membrane represent the basic requirement for the PMA-dependent cytolytic process, whereas MPO behaves as a device to amplify lysis.
TI - Cellular cytotoxicity mediated by granule-depleted neutrophil cytoplasts
JF - Annals of Hematology
DO - 10.1007/BF00320117
DA - 1985-08-01
UR - https://www.deepdyve.com/lp/springer-journals/cellular-cytotoxicity-mediated-by-granule-depleted-neutrophil-trNv0ScLh0
SP - 97
EP - 102
VL - 51
IS - 2
DP - DeepDyve
ER -