TY - JOUR
AU - Iseri, Lloyd T.
AB - TECHNICAL SECTION TH E QUANTITATIVE ESTIMATION OF CALCIUM IN HUMAN PLASMA BY FLAME SPECTROPHOTOMETRY* ROBERT E. MOSHER, Pir.D.,f MASASHI ITANO, B.S., A. J. BOYLE, M.D., GORDON B. MYERS, M.D., AND LLOYD T. ISERI, M.D. From the Departments of Chemistry and Medicine, Wayne University, Detroit, Michigan This communication deals with the use of the Beckman spectrophotometer, Model DU, and Beckman flame photometer attachment for the determination of calcium in human plasma. The procedure involves: (1) preliminary treatment of the sample with nitric-perchloric acid mixture and evaporation to incipient dryness in order to destroy plasma protein and to free bound-calcium; (2) solution in hydrochloric acid, transfer to a centrifuge tube and precipitation as tricalcium phosphate by diammonium hydrogen phosphate in the presence of excess ammo­ nium hydroxide; (3) isolation of calcium phosphate by centrifugation, discard of the supernate, and solution of the precipitate with hydrochloric acid and dilution to a fixed volume with a solution containing a known amount of sodium; (4) uti­ lization of standard calcium solutions containing sodium and phosphate in amounts similar to the prepared sample in order to correct for enhancement of calcium spectral bands caused by sodium and the depression caused by phos­ phate; (5) comparison 
TI - The Quantitative Estimation of Calcium in Human Plasma by Flame Spectrophotometry*
JF - American Journal of Clinical Pathology
DO - 10.1093/ajcp/21.1_ts.75
DA - 1951-01-01
UR - https://www.deepdyve.com/lp/oxford-university-press/the-quantitative-estimation-of-calcium-in-human-plasma-by-flame-tUh3TVNbSt
SP - 75
EP - 80
VL - 21
IS - 1_ts
DP - DeepDyve
ER -