TY - JOUR
AU - Hase,, Sumihiro
AB - Abstract Type and structural analysis of O-linked sugar chains in human blood clotting factor IX was performed by the pyridylamination method developed for O-linked sugar chains [Kuraya, N. & Hase, S. (1992) J. Biochem 112, 122–126]. O- and N-linked sugar chains were released with hydrazine, and then N-acetylated, followed by pyridylamination. The type of sugar chain was determined by reducing-end analysis of the pyridylaminated (PA-) sugar chains. Sugar chains with PA-GalNAc at the reducing terminal and that with PA-Fuc [Nishimura, H. et al. (1992) J. Biochem 267, 17520–17525] were obtained besides known sugar chains with PA-Glc from the Xyl-Glc-Ser type and those with PA-GlcNAc from asparagine-linked sugar chains. The sugar chains with PA-GalNAc were identified as mono- and disialyl Galβl-3GalNAc by two-dimensional HPLC mapping. The structure of the sugar chain with PA-Fuc was Neu5Acα2–6Galβl-4GlcNAcβ1-3Fuc, as determined by exoglycosidase digestion, raethylation analysis, and Smith degradation. This content is only available as a PDF. Author notes 1This study was supported in part by Special Coordination Funds from the Science and Technology Agency of the Japanese Government. 2Present address: Faculty of Pharmaceutical Sciences, Hokkaido University, Kira-kn, Sapporo 060 © 1993 BY THE JOURNAL OF BIOCHEMISTRY
TI - Structural Analysis of O-Linked Sugar Chains in Human Blood Clotting Factor IX
JF - The Journal of Biochemistry
DO - 10.1093/oxfordjournals.jbchem.a124252
DA - 1993-12-01
UR - https://www.deepdyve.com/lp/oxford-university-press/structural-analysis-of-o-linked-sugar-chains-in-human-blood-clotting-tSgODMx38e
SP - 763
EP - 765
VL - 114
IS - 6
DP - DeepDyve
ER -