TY - JOUR
AU - Jakobsen, Iver
AB - An open flow‐through gas system was used to investigate the effect of plant age on nitrogenase activity in relation to root respiration (measured as CO2 release) and supra‐ambient O2 levels in 24‐ to 51‐day‐old, nodulated Pisum sativum L. cv. Bodil. The effect of assaying plants repeatedly was also studied. The respiratory efficiency of nitrogenase [mol CO2 (mol C2H4)−1] and the relative decline in nitrogenase (EC 1.7.99.2) activity in response to introduction of C2H2 in the gas stream were unaffected by plant age. In contrast, the nitrogenase‐linked respiration as a proportion of total root respiration increased with time. Accordingly, the specific respiration linked‐to growth and maintenace of the noduled root system decreased with time. C2H2 reduction and root respiration were increased by supra‐ambient O2 levels, but the tolerance to high O2 concentrations seemed to decrease with plant age. Repeated C2H2 assays on the same plants decreased their rate of growth and N accumulation: in addition, nitrogenase activity and root respiration were somewhat negatively affected. The results indicate that results from experiments with plants of different ages cannot always be directly compared, and that repeated C2H2 assays on the same plants should be applied with caution in physiological work.
TI - Effects of age, supra‐ambient oxygen and repeated assays on acetylene reduction and root respiration in pea
JO - Physiologia Plantarum
DO - 10.1111/j.1399-3054.1988.tb04944.x
DA - 1988-01-01
UR - https://www.deepdyve.com/lp/wiley/effects-of-age-supra-ambient-oxygen-and-repeated-assays-on-acetylene-tKPkJ0nRVd
SP - 77
EP - 82
VL - 74
IS - 1
DP - DeepDyve
ER -