TY - JOUR
AU - Furuichi, Yasushi
AB - The purpose of this study was to improve the adhesion and extension of human gingival epithelial cells (HGECs) to the yttria‐stabilized zirconia polycrystal (Y‐TZP) surfaces by immobilization of insulin‐like growth factor 1 (IGF‐1). Surface analyses by Fourier transform infrared spectroscopy (FTIR) and X‐ray photoelectron spectroscopy (XPS) showed that IGF‐1 was successfully immobilized on the Y‐TZP surfaces. There was no significant difference between the number of cells attached to the IGF‐1‐immobilized Y‐TZP surfaces and on the as‐polished Y‐TZP surfaces either at 3 or 72 h. However, IGF‐1‐immobilized Y‐TZP surfaces yielded a significantly higher expression of integrin β4 mRNA and laminin‐5 mRNA, and enhanced adhesion strength of HGECs after 72 h of incubation. There was no difference between the amount of adhered Streptococcus gordonii (S. gordonii) found on the IGF‐1‐immobilized Y‐TZP surfaces and on the as‐polished Y‐TZP surfaces. These results suggested that the IGF‐1‐immobilized Y‐TZP surfaces developed using the method reported herein enhanced the adhesion and extension of HGECs to the Y‐TZP surfaces without enhancing S. gordonii adhesion. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3659–3665, 2015.
TI - Biological activation of zirconia surfaces by chemical modification with IGF‐1
JO - Journal of Biomedical Materials Research Part A
DO - 10.1002/jbm.a.35476
DA - 2015-11-01
UR - https://www.deepdyve.com/lp/wiley/biological-activation-of-zirconia-surfaces-by-chemical-modification-sv2aV8msJ9
SP - 3659
EP - 3665
VL - 103
IS - 11
DP - DeepDyve
ER -