TY - JOUR
AU - Litwin, .Joanna
AB -  P. CZERSKI,S. SZMICIELSKI and JOANNA LITWIN Ikpartnient of Histology and Emhyology, University Medical Scliool, and I)c*parttnent of Clinical Hematology, State Institute of Hematology, War5aw The growing interest in the physiology of lymphocytes caused the need for obtaining pure suspensions of these cells from peripheral blood. Numerous ingenious methods were devised t o this end [5]. I n the course of our experiments on lymphocyte cultivations in vitro none of these methods was found satisfactory for routine use, not in our hands a t least. Most promising simple methods previously described [2, 41 were tested and finally a routine procedure was developed which fulfilled the following requirements : (1) The suspension should be contaminated as little as possible with other cells. (2) The apparatus which is necessary must be as simple as possible. (3) The handling of the cells must be limited t o a minimum. (4) The maintenance of sterile conditions should be easily possible. (5) N o cytotoxic agents must be introduced. Basic principles. Filtration of leukocyte suspensions through glass wool columns [2] or sedimentation in glass containers [4] eliminates platelets, granulocytes and monocytes due t o their adhesion t o foreign surfaces. The observation of the 
TI - Simple Methods for Obtaining Purified Suspensions of Lymphocytes
JF - Vox Sanguinis
DO - 10.1111/j.1423-0410.1966.tb04278.x
DA - 1966-11-12
UR - https://www.deepdyve.com/lp/wiley/simple-methods-for-obtaining-purified-suspensions-of-lymphocytes-soFye3TOhK
SP - 734
VL - 11
IS - 6
DP - DeepDyve
ER -