TY - JOUR
AU - ISHIBASHI,, Sadahiko
AB - Abstract A difference in the mode of inhibition of hexokinase [EC 2.7.1.1] isoenzymes by pChloromercuribenzenesulfonate was confirmed with respect to glucose between two Type I isoenzyme preparations purified from the kidney and spleen of rat. Essentially the same difference was observed when galactose was used as the substrate in place of glucose, as the kidney Type I isoenzyme was inhibited in a competitive manner while the spleen counterpart was inhibited in a non-competitive manner by sulfhydryl inhibitor. Both the Type I isoenzymes, however, were competitively inhibited by other mercurial sulfhydryl inhibitors, methyl and butyl mercuric chlorides. On the other hand, the Type II hexokinase isoenzymes purified from the muscle, heart, and spleen were all inhibited competitively by P-chloromercuribenzenesulfonate with respect to glucose. The mechanism of competitive inhibition of the hexokinase isoenzymes by sulfhydryl inhibitors was discussed in view of the difference in the mode of action of the mercurials with different isoenzymes. This content is only available as a PDF. © 1976 BY THE JOURNAL OF BIOCHEMISTRY
TI - Competitive Inhibition of Hexokinase Isoenzymes by Mercurials
JF - The Journal of Biochemistry
DO - 10.1093/oxfordjournals.jbchem.a131098
DA - 1976-03-01
UR - https://www.deepdyve.com/lp/oxford-university-press/competitive-inhibition-of-hexokinase-isoenzymes-by-mercurials-slNPhqJSPr
SP - 543
EP - 548
VL - 79
IS - 3
DP - DeepDyve
ER -