TY - JOUR
AU1 - Maeki, Masatoshi
AU2 - Ishida, Akihiko
AU3 - Tokeshi, Manabu
AB - Three-dimensional protein structure determination by X-ray crystallography is essential for understanding biological function and accelerating drug discovery. However, obtaining high-quality protein crystals remains a significant bottleneck. The conventional crystallization methods are often labor-intensive, require large sample volumes, and offer limited control over the crystallization environment. This review summarizes the application of microfluidic technologies to protein crystallography with a focus on their advantages over the conventional crystallization methods. Microfluidic devices enable nanoliter-scale sample handling, precise control over crystallization conditions, and high-throughput screening, addressing major limitations of the conventional approaches. This review introduces various microfluidic platforms, including droplet-based and microwell-based systems, for protein crystallization, crystal growth control, and on-chip X-ray diffraction analysis. The review also covers the use of microfluidics for creating diffusion-controlled crystal growth environments, real-time crystal growth measurement, on-chip X-ray diffraction measurement, and room-temperature X-ray crystallography with automated data processing.Graphical abstract[graphic not available: see fulltext]
TI - Microfluidic technologies for protein crystallography: advances and applications
JF - Analytical Sciences
DO - 10.1007/s44211-025-00767-z
DA - 2025-04-21
UR - https://www.deepdyve.com/lp/springer-journals/microfluidic-technologies-for-protein-crystallography-advances-and-saur7JkqiC
SP - 1
EP - 9
VL - OnlineFirst
IS - 
DP - DeepDyve
ER -