TY - JOUR
AU - NUMANO, FUJIO
AB - Inhibition of Human Vascular Smooth Muscle Cell Proliferation by Interferon Gamma KENTARO SHIMOKADO AND FUJI0 NUMANO Third Department of Internal Medicine Tokyo Medical and Dental University 1-5-45 Yushima, Bunkyo-ku Tokyo 113, Japan Although T lymphocytes are present in the atherosclerotic lesion, their roles in athero- genesis are still not clear. To elucidate the potential role of the T lymphocyte, we investigated the effects of interferon gamma (INF), a product of activated lymphocytes, on smooth muscle cell proliferation. METHODS Human arterial smooth muscle cells (HUA-SMCs) were prepared from human um- bilical artery by the method of R. Ross and were maintained in RPMI 1640 medium supplemented with 20% FCS, 10 ng/ml basic FGF. The third passage of HUA-SMCs was seeded in a 24-well tissue culture dish (2 cm*/well) and cultured in regular medium with or without human recombinant interferon gamma (provided by Takeda Pharmaceutical Co.) for 3-5 days. Cells were trypsinized with 0.25% trypsin/EDTA and the cell number was determined with a Coulter particle counter. RESULTS AND DISCUSSION Under our experimental conditions, HUA-SMCs grew logarithmically for 12-1 5 days and became confluent approximately at 2 x 10’ cells/cm2. When they were cultured in the presence of 0.1-100 u./ml INF, the 
TI - Inhibition of Human Vascular Smooth Muscle Cell Proliferation by Interferon Gamma
JF - Annals of the New York Academy of Sciences
DO - 10.1111/j.1749-6632.1990.tb42340.x
DA - 1990-01-01
UR - https://www.deepdyve.com/lp/wiley/inhibition-of-human-vascular-smooth-muscle-cell-proliferation-by-sWpHlO25Pp
SP - 544
EP - 545
VL - 598
IS - 1
DP - DeepDyve
ER -