TY - JOUR
AU1 - Han, Donghoon
AU2 - Kim, Yang-Rae
AU3 - Oh, Jeong-Wook
AU4 - Kim, Tae Hyun
AU5 - Mahajan, Rakesh Kumar
AU6 - Kim, Jong Seung
AU7 - Kim, Hasuck
AB - We have developed a selective, sensitive, and re-usable electrochemical sensor for Hg2+ ion detection. This sensor is based on the Hg2+-induced conformational change of a single-stranded DNA (ssDNA) which involves an electroactive, ferrocene-labeled DNA hairpin structure and provides strategically the selective binding of a thymine–thymine mismatch for the Hg2+ ion. The ferrocene-labeled DNA is self-assembled through S–Au bonding on a polycrystalline gold electrode surface and the surface blocked with 3-mercapto-1-propanol to form a mixed monolayer. The modified electrode showed a voltammetric signal due to a one-step redox reaction of the surface-confined ferrocenyl moiety. The ‘signal-on’ upon mercury binding could be attributed to a change in the conformation of ferrocene-labeled DNA from an open structure to a restricted hairpin structure. The differential pulse voltammetry (DPV) of the modified electrode showed a linear response of the ferrocene oxidation signal with increase of Hg2+ concentration in the range between 0.1 and 2 µM with a detection limit of 0.1 µM. The molecular beacon mercury(ii) ion sensor was amenable to regeneration by simply unfolding the ferrocene-labeled DNA in 10 µM cysteine, and could be regenerated with no loss in signal gain upon subsequent mercury(ii) ion binding.
TI - A regenerative electrochemical sensor based on oligonucleotide for the selective determination of mercury(ii)
JF - The Analyst
DO - 10.1039/b908457f
DA - 2009-08-17
UR - https://www.deepdyve.com/lp/royal-society-of-chemistry/a-regenerative-electrochemical-sensor-based-on-oligonucleotide-for-the-s4QZ2LceZ0
SP - 1857
EP - 1862
VL - 134
IS - 9
DP - DeepDyve
ER -