TY - JOUR
AU1 - Ohe, Joo-Young
AU2 - Choi, Byung-Joon
AU3 - Jung, Junho
AU4 - Chun, Jeewan
AU5 - Yoon, Youngjae
AU6 - Kwon, Yong-Dae
AB - Abstract The purpose of this research was to examine the effect of biphasic hydroxyapatite and b-tricalcium phosphate (HAp-bTCP) immobilized GDF-5 to assess the hypothesis that osteoblast is enhanced and bone formation is promoted when compared to biphasic HAp-bTCP in vitro and in vivo. First, we characterized the release profiles from HAp-bTCP and tested their cell viability, differentiation. After that, we loaded GDF-5 to HAp-bTCP. In the animal study, 4 full thickness critical-size calvarial defects were prepared in a total of 16 male rabbits. The cranial defects were filled with HAP-bTCp, or heparized HAP-bTCP. At 5 and 10 weeks, dissected specimens were processed for histological and histochemical analysis. GDF-5 was considered to be released in a sustained mode from HAp-bTCP. In vitro results provided that the proliferation and differentiation of MG-63 cells to HAp-bTCP-GDF-5 was higher than that of the control group. In addition, in vivo animal studies showed that HAp-bTCP-GDF-5 has a significant improvement to bone formation. HAp-bTCP-GDF-5 is thought to be an excellent biomaterial that delivers osteogenic differentiation factors such as GDF-5, and GDF-5 can be possibly useful as an effective alternative to avail new bone formation.
TI - Osteoblastic Differentiation of Functionalized Biphasic Hydroxyapatite and b-Tricalcium Phosphate with Recombinant Human Growth and Differentiation (rhGDF-5)
JF - "Macromolecular Research"
DO - 10.1007/s13233-018-6142-9
DA - 2018-11-01
UR - https://www.deepdyve.com/lp/springer-journals/osteoblastic-differentiation-of-functionalized-biphasic-hydroxyapatite-s2swaf5GMN
SP - 1011
EP - 1018
VL - 26
IS - 11
DP - DeepDyve
ER -