TY - JOUR
AU - Sternesjö, Åse
AB - Abstract Two recently developed surface plasmon resonance biosensor assays for detection of β-lactams in milk were used to screen raw producer milk samples. Both assays use a β-lactam receptor protein with carboxypeptidase activity for detection. The results of the biosensor assays were compared with those of various commercial screening tests, i.e., the Delvotest SP, Penzym S, Beta-STAR, SNAP, and Parallux. The results of the 2 biosensor assays showed good agreement with those of the other screening tests. Of 195 analyzed milk samples, the results of only 5 samples differed between the assays. Additionally, 30 milk samples with both negative and positive results in the screening assays were analyzed by liquid chromatography for identification and quantification of any β-lactam residues. All screening tests showed 0% false-negative results with 15 incurred samples containing between 4.0 and 268 μg/kg penicillin G. The biosensor assays showed 27% positive results (false violatives) with 15 producer milk samples containing penicillin G concentrations between 0 and 3.6 μg/kg, i.e., below maximum residue limit. This figure varied between 27 and 53% for the other screening tests. This content is only available as a PDF. © Journal of AOAC International This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) © Journal of AOAC International
TI - Biosensor Analysis of β-Lactams in Milk: Comparison with Microbiological, Immunological, and Receptor-Based Screening Methods
JF - Journal of AOAC INTERNATIONAL
DO - 10.1093/jaoac/87.3.614
DA - 2004-05-01
UR - https://www.deepdyve.com/lp/oxford-university-press/biosensor-analysis-of-lactams-in-milk-comparison-with-microbiological-rpsNBPrCRN
SP - 614
EP - 620
VL - 87
IS - 3
DP - DeepDyve
ER -