TY - JOUR
AU - Sadèe,, W
AB - Abstract We measured procainamide (I) and its metabolite, N¬acetylprocainamide (II), in human serum samples by sol¬vent extraction, high-performance liquid chromatography on a reverse phase column, and detection at 280 nm, with use of external standards. The method requires 0.2 ml of serum and is sensitive to 0.3 mg of I and 0.6 mg of II per liter of serum, with intra-assay standard deviations of 0.22 and 0.24 mg/liter, respectively, at 5 mg/liter (n = 10) and inter-assay standard deviations of 0.63 and 0.81 mg/liter, respectively, at 7.5 mg/liter (CV 8.4 and 10.5 % , respec¬tively, n = 20). Concentrations measured by high-perfor¬mance liquid chromatography and by an established flu¬orescence technique correlated well (r = 0.98 for I and 0.97 for II). No interfering substances were found in 20 randomly selected sera from patients receiving a large number of other drugs. Of the pure drug substances tested, only sulfathiazole interfered with the assay of II. The method is therefore suitable for routinely monitoring these compounds in serum in a clinical laboratory. The high concentrations of the metabolite in a significant number of patients demonstrate the need to consider it as well as the parent drug as guides in optimizing dosage regiments for I. drug assay , assay of an active drug metabolite This content is only available as a PDF. © 1958 The American Association of Clinical Chemists, Inc. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
TI - Measurement of Procainamide and N-Acetylprocainamide in Serum by High-Performance Liquid Chromatography
JO - Clinical Chemistry
DO - 10.1093/clinchem/23.4.636
DA - 1977-04-01
UR - https://www.deepdyve.com/lp/oxford-university-press/measurement-of-procainamide-and-n-acetylprocainamide-in-serum-by-high-rly58Pw9j8
SP - 636
EP - 638
VL - 23
IS - 4
DP - DeepDyve
ER -