TY - JOUR
AU - Coleman,, Geoffrey
AB - Abstract Although considerable homology exists between the translation products of the rplL, rpoB and rpoC genes of the β operons of the Gram-negative organism Escherichia coli and the Gram-positive Staphylococcus aureus the region between the rplL and rpoB genes is quite different in the two bacterial species. In E. coli the 324 bp has three centres of dyad symmetry in the first half of the sequence and multiple nonsense codons in all three reading frames. By contrast, the corresponding region in S. aureus consists of 1000 bp capable of forming a similar arrangement of stem-loop structures but with an open reading frame, sited 177 bp downstream of the end of rplL and 217 bp upstream of the beginning of the rpoB gene, with consensus initiation and termination signals, which if translated would generate a 22,665 Da protein with 202 amino acids. In view of the inability to find any significant homology with other proteins in the data bank and because the evidence suggests, as in E. coli, that the rplL-rpoB intergenic sequence is involved in regulation it is proposed that the expression product of orf202 may be a further element of control in the S. aureusβ operon. Staphylococcus aureus, Nucleotide sequence, orf202, Novel protein, β operon References [1] Moorman D.R Mandel G.L ( 1981 ) Characteristics of rifampin-resistant variants obtained from clinical isolates of Staphylococcus aureus Antimicrob. Agents Chemother. , 20 , 709 – 713 . Google Scholar Crossref Search ADS PubMed WorldCat [2] Al-Ani B Aboshkiwa M Glass R Coleman G ( 1991 ) The patterns of extracellular protein formation by spontaneously occurring rifampicin-resistant mutants of Staphylococcus aureus FEMS Microbiol. Lett. , 70 , 91 – 94 . Google Scholar Crossref Search ADS WorldCat [3] Jin D.J Gross C.A ( 1989 ) Characterization of the pleiotropic phenotypes of rifampicin-resistant rpoB mutants of Escherichia coli J. Bacteriol. , 171 , 5229 – 5231 . Google Scholar Crossref Search ADS PubMed WorldCat [4] Abbas-Ali B Coleman G ( 1977 ) The characteristics of extracellular protein secretion by Staphylococcus aureus (Wood 46) and their relationship to the regulation of α-toxin formation J. Gen. Microbiol. , 99 , 277 – 282 . Google Scholar Crossref Search ADS PubMed WorldCat [5] Janzon L Lofdahl S Arvidson S ( 1986 ) Evidence for a coordinate transcription control of alpha-toxin and protein A in Staphylococcus aureus FEMS Microbiol. Lett. , 33 , 193 – 198 . Google Scholar Crossref Search ADS WorldCat [6] Vandenesch F Komblum J Novick R.P ( 1991 ) A temporal signal, independent of agr, is required for hla but not spa transcription in Staphylococcus aureus J. Bacteriol. , 173 , 6313 – 6320 . Google Scholar Crossref Search ADS PubMed WorldCat [7] Aboshkiwa M Al-Ani B Coleman G Rowland G ( 1992 ) Cloning and physical mapping of the Staphylococcus aureus rplL, rpoB and rpoC genes, encoding ribosomal protein L7/L12 and RNA polymerase subunits β and b' J. Gen. Microbiol. , 138 , 1875 – 1880 . Google Scholar Crossref Search ADS PubMed WorldCat [8] Jinks-Robertson S Nomura M ( 1987 ) In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology Ribosomes and tRNA , Neidhardt F.C, Ed) pp 1358 – 1385 American Society of Microbiology , Washington, DC. Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC [9] Post L.E Strycharz G.D Nomura M Lewis H Dennis P.P ( 1979 ) Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit β in Escherichia coli 76 , In Proc. Natl. Acad. Sci. USA , pp 1697 – 1701 . [10] Mead D.A Szczesna-Skorupa E Kemper B ( 1986 ) Single stranded DNA ‘blue’ T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering Prot. Eng. , 1 , 67 – 74 . Google Scholar Crossref Search ADS WorldCat [11] Sanger F Nicklen S Coulson A.R ( 1977 ) DNA sequencing with chain-terminating inhibitors 74 , In Proc. Natl. Acad. Sci. USA , pp 5463 – 5467 . [12] Henikoff S ( 1984 ) Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing Gene , 28 , 351 – 359 . Google Scholar Crossref Search ADS PubMed WorldCat [13] McLaughlin J.R Murray C.L Rabinowitz J.C ( 1981 ) Unique features in the ribosome binding site sequence of the Gram-positive Staphylococcus aureusβ-lactamase gene J. Biol. Chem. , 256 , 11283 – 11291 . Google Scholar PubMed OpenURL Placeholder Text WorldCat [14] Shine J Dalgarno L ( 1974 ) The 3′ termination sequence of Escherichia coli 16S ribosomal RNA complementary to nonsense triplets and ribosome binding sites 71 , In Proc. Natl. Acad. Sci. USA , pp 1342 – 1346 . [15] Pribnow D ( 1975 ) Bacteriophage T7 early promoter nucleotide sequences of two RNA polymerase binding sites J. Mol. Biol. , 99 , 419 – 443 . Google Scholar Crossref Search ADS PubMed WorldCat [16] Deuschle U Kammerer W Gentz R Bujard H ( 1986 ) Promoters of Escherichia coli: a hierarchy of in vivo strength indicates alternate structures EMBO J. , 5 , 2987 – 2994 . Google Scholar PubMed OpenURL Placeholder Text WorldCat [17] Kupper H Sekiya T Rosenberg M Egan J Landy A ( 1978 ) A r-dependent termination site in the gene coding for tyrosine tRNA su3 of Escherichia coli Nature , 272 , 423 – 428 . Google Scholar Crossref Search ADS PubMed WorldCat [18] Carafa Y.d'A Brody E Thermes C ( 1990 ) Prediction of rho-independent Escherichia coli transcription terminators. A statistical analysis of their RNA stem-loop structures J. Mol. Biol. , 216 , 835 – 858 . Google Scholar Crossref Search ADS PubMed WorldCat [19] Cozens A.L Walker J.E ( 1987 ) The organization and sequence of the genes for ATP synthase subunits in the cyanobacterium Synechococcus 6301. Support for an endosymbiotic origin of chloroplasts J. Mol. Biol. , 194 , 359 – 383 . Google Scholar Crossref Search ADS PubMed WorldCat [20] Armstrong G.A Alberti M Leach F Hearst J.E ( 1989 ) Nucleotide sequence, organization and nature of the protein products of the carotinoid biosynthesis gene cluster of Rhodobacter capsulatus Mol. Gen. Genet. , 216 , 254 – 268 . Google Scholar Crossref Search ADS PubMed WorldCat This content is only available as a PDF. © 1995 Federation of European Microbiological Societies. All rights reserved
TI - Nucleotide and deduced amino acid sequence of the gene for a novel protein with a possible regulatory function encoded in the β operon of Staphylococcus aureus
JF - FEMS Microbiology Letters
DO - 10.1111/j.1574-6968.1995.tb07434.x
DA - 1995-03-01
UR - https://www.deepdyve.com/lp/oxford-university-press/nucleotide-and-deduced-amino-acid-sequence-of-the-gene-for-a-novel-rVw0VBt0Zr
SP - 305
EP - 309
VL - 126
IS - 3
DP - DeepDyve
ER -