TY - JOUR
AU - Châteaureynaud,, Pierrette
AB - Abstract In 15 pregnant women during the third term of pregnancy, the immunomodulatory property of α2-macroglobulin (α2M) was initially detected by measuring the inhibitory effect on immune complement-dependent haemolysis of serum α2M fractions obtained by gel filtration. By a two-step chromatography procedure consisting of gel filtration followed by anion-exchange chromatography, different sub-forms of α2M in serum were separated. Amongst them, it was shown that the inhibition of complement activity was almost exclusively linked to one particular subform. Additional studies revealed that the observed effect was not due to proteases bound to α2M during clotting since, by using protease-specific inhibitors, no change was observed in complement inhibition. This subform, though present at very low levels in control sera, appeared in strikingly increased amounts during the third trimester of pregnancy (35 mg/1) and comprised between 3 and 5% of the total α2M. Results show that the increase of α2M anticomplementary activity is linked to the increase in α2M levels in serum. anticomplementary activity, human, α2-macroglobulin, pregnancy This content is only available as a PDF. © Oxford University Press
TI - Characterization of one α2-macroglobulin with anticomplementary activity from pregnant women
JF - Human Reproduction
DO - 10.1093/oxfordjournals.humrep.a137180
DA - 1990-08-01
UR - https://www.deepdyve.com/lp/oxford-university-press/characterization-of-one-2-macroglobulin-with-anticomplementary-qlXEJ9t1bB
SP - 747
EP - 754
VL - 5
IS - 6
DP - DeepDyve
ER -