TY - JOUR
AU1 - Tesarik,, Jan
AB - Abstract This study was undertaken to determine the site of the acrosome reaction of spermatozoa penetrating into freshly inseminated human oocytes. The inseminated oocytes were treated with an anti-acrosin monoclonal antibody and the bound antibody was visualized at the ultrastructural level with the use of a second peroxidase-conjugated antibody. Quantitative analysis of serial thin sections cut throughout the specimens showed that the number of spermatozoa within the zona pellucida (potentially fertilizing ones) corresponded to the number of acrosin deposits associated with acrosomal ghosts on the zona pellucida surface. As it is known that a large acrosin bundle is liberated from a spermatozoon at a well-defined point of the acrosome reaction, these findings indicate that the acrosome reaction of the fertilizing spermatozoon must be exactly synchronized with its penetration through the egg vestments, apparently by the action of specific acrosome reaction-promoting substances in the oocyte/cumulus complex. These results represent a theoretical basis for evaluation of direct and indirect laboratory tests for human sperm acrosome reaction. ‘Good’ sperm samples should display elevated levels of acrosome-reacted spermatozoa after the administration of an appropriate stimulus and low levels of spontaneous acrosome reactions. acrosome raction, human sperm, acrosin, egg vestments This content is only available as a PDF. Author notes 1Presented address: INSERM Unité 187, Service de Gynécologie-Obstétrique, Hôpital Antoine-Béclère, 92141 Clamart, France © Oxford University Press
TI - Appropriate timing of the acrosome reaction is a major requirement for the fertilizing spermatozoon
JF - Human Reproduction
DO - 10.1093/oxfordjournals.humrep.a137020
DA - 1989-11-01
UR - https://www.deepdyve.com/lp/oxford-university-press/appropriate-timing-of-the-acrosome-reaction-is-a-major-requirement-for-q50OZtX1js
SP - 957
EP - 961
VL - 4
IS - 8
DP - DeepDyve
ER -