TY - JOUR
AU - Hase, Sumihiro
AB - Abstract Cytosolic neutral α-mannosidase is a putative catabolic enzyme that produces cytosolic free oligomannosides. Activation of the enzyme by Cod(II) treatment has been reported using pyridylamino derivatives of Man5 GlcNAc and MangGlcNAcj, and p-nitrophenyl α-mannoside as substrates, with the Cod(II)-treated enzyme releasing four a-mannose residues from Mai9GlcNAc to give Manα1;6(Manαl-2Manal-2Manal-3)Ma^l-4GlcNAc as an end product. When Man9GlcNAc, which is considered to be the actual substrate in the cytosol, was used as a substrate, we found that even before treatment with Co(II) the enzyme was able to cleave a single Manα1-2 residue from Man9GlcNAc to give Manα1-6(Manα1-2Manα1-3)Manα1-6(Manα1-2Manα1-2Manα1-3)Manβ1-4GlcNAc as the end product. The Km value of the Cod(II)-treated enzyme for Man9GlcNAc was found to be 37 μM, which is one-twelfth that of the non-treated enzyme, while the values were Vmax values were almost the same, indicating that the affinity of the substrate is higher with Cod(II). These results indicate that Cod(II) regulates the substrate specificity of the enzyme. cytosol, a-mannosidase, Cod(II), free oligosaccharides © 2002 by the Japanese Biochemical Society
TI - Co(II)-Regulated Substrate Specificity of Cytosolic α-Mannosidase
JO - The Journal of Biochemistry
DO - 10.1093/oxfordjournals.jbchem.a003218
DA - 2002-08-01
UR - https://www.deepdyve.com/lp/oxford-university-press/co-ii-regulated-substrate-specificity-of-cytosolic-mannosidase-pRoYptiCLF
SP - 253
EP - 256
VL - 132
IS - 2
DP - DeepDyve
ER -