TY - JOUR
AU - Evans, Charles A.
AB - CULTIVATION OF MOUSE-ADAPTED DENGUE VIRUS (TYPE 1) IN RHESUS MONKEY TISSUE CULTURE SUSUMU HOTTA* AND CHARLES A. EVANS From the Department of Microbiology, University of Washington, Seattle, Washington second consisted of trypsin-treated kidney cells Although dengue virus has been suc­ grown directly on a glass surface and incubated cessfully adapted to mice, attempts to in the stationary state (Youngner, 1954). Either cultivate it in tissue culture have so far screw-capped or rubber-stoppered tubes, 16 X 150 yielded only negative or questionable mm, were used. Following incubation at 37 C results (Sabin, 1952; Hotta, 1952). The for 4 to 5 days, tubes showing good cellular growth were selected for viral inoculation. Occasionally, only reported exception is contained in a they were stored at 10 or 20 C for a period not short discussion by Schlesinger (1950) of exceeding 1 week prior to inoculation with virus. experiments in which dengue virus was Medium.-For the initiation of cellular growth, propagated in 5-day-old chick embryo or the storage of well-grown cultures, medium D tissue. t In the present paper experi­ of Youngner (mixture 199 with 2% inactivated horse serum) was used. The culture medium used ments are reported in which two strains after 
TI - Cultivation of Mouse-adapted Dengue Virus (Type 1) in Rhesus Monkey Tissue Culture
JO - The Journal of Infectious Diseases
DO - 10.1093/infdis/98.1.88
DA - 1956-01-01
UR - https://www.deepdyve.com/lp/oxford-university-press/cultivation-of-mouse-adapted-dengue-virus-type-1-in-rhesus-monkey-p5lS3fLv41
SP - 88
EP - 97
VL - 98
IS - 1
DP - DeepDyve
ER -