TY - JOUR
AU - Motto, Mario
AB - The maize Opaque-2 (O2) protein is a transcription factor of the basic/leucine-zipper class, involved in the regulation of endosperm proteins including the 22 kDaα-zein storage proteins and b32 protein. In this study we have focussed our attention on the relationship betweenO2 and thecyPPDK1 gene, which encodes a cytoplasmic pyruvate orthophosphate dikinase (PPDK) isoform. The results of this study showed that PPDK activity is detectable in wild-type maize endosperms, while ino2 mutant endosperms, the levels of PPDK protein, mRNA, and enzymatic activity are reduced, indicating thatO2 is involved in the regulation ofcyPPDK1 in this tissue. By employing transient expression experiments in tobacco mesophyll protoplasts, we have demonstrated that the O2 protein can activate expression of a chloramphenicol acetyl transferase reporter gene placed under the control of thecyPPDK1 promoter. An in vitro binding assay and DNaseI footprint analysis demonstrated that a specific sequence in thecyPPDK1 promoter can be recognized and protected by maize O2 protein. The regulation by theO2 locus ofcyPPDK1 reported here, and control ofα-zein synthesis byO2 suggest that the O2 protein may play a more general role in maize endosperm development than previously thought.
TI - The transcriptional activatorOpaque-2 controls the expression of a cytosolic form of pyruvate orthophosphate dikinase-1 in maize endosperms
JO - Molecular Genetics and Genomics
DO - 10.1007/BF02174452
DA - 2005-08-31
UR - https://www.deepdyve.com/lp/springer-journals/the-transcriptional-activatoropaque-2-controls-the-expression-of-a-oTsgU7xav4
SP - 647
EP - 654
VL - 250
IS - 5
DP - DeepDyve
ER -