TY - JOUR
AU - FUKUWATARI, Tsutomu
AB - Abstract 2-Oxoadipic acid, a key metabolite of tryptophan and lysine, reacted with 1,2-diamino-4,5-methylenebenzene in an acidic solution to produce a fluorescent derivative. The reaction product was separated using a Tosoh ODS-80Ts column with 20 mmol/L of KH2PO4–K2HPO4 buffer (pH 7.0) containing 26% methanol at a flow rate 0.8 mL/min. The excitation wavelength of detection was 367 nm, and the emission wavelength was 446 nm. The limit of quantification was 1 pmol per injection, sufficiently sensitive for the determination of 2-oxoadipic acid in human and experimental animal urine. 2-oxoadipic acid, 1,2-diamino-4,5-methylenebenzene, tryptophan, lysine, urine Reference 1) Shibata K and Matsuo H, J. Nutr., 119, 896–901 (1989). 2) Egashira Y, Nakazawa A, Ohta T, Shibata K, and Sanada H, Comp. Biochem. Physiol., 111, 539–545 (1995). 3) Fukuwatari T, Ohsaki S, Fukuoka S, Sasaki R, and Shibata K, Toxicol. Sci., 81, 302–308 (2004). 4) Tanabe A, Egashira Y, Fukuoka S, Shibata K, and Sanada H, J. Nutr., 132, 1153–1159 (2002). 5) Fukuoka S, Ishiguro K, Yanagihara K, Tanabe A, Egashira Y, Sanada H, and Shibata K, J. Biol. Chem., 277, 35162–35167 (2002). 6) Fukuwatari T, Ohta M, Sugimoto E, Sasaki R, and Shibata K, Biochim. Biophys. Acta, 1672, 67–75 (2004). 7) Przyrembel H, Bachmann D, Lombeck I, Becker K, Wendel U, and Wadman SK, Clin. Chim. Acta, 58, 257–269 (1975). 8) Xia ZW, Inoue Y, Ohse M, Shinka T, and Kuhara T, World J. Gastroenterol., 6, 766–769 (2000). 9) Matsumoto I, Kuhara T, Inoue Y, Shinka T, and Matsumoto I, Biomed. Environ. Mass Spectrom., 15, 43–57 (1996). 10) Nakamura M, Hara S, Yamaguchi M, Takemori Y, and Ohkura Y, Chem. Pharm. Bull., 35, 687–692 (1987). 11) Fukuwatari T, Suzuki Y, Sugimoto E, and Shibata K, Biosci. Biotechnol. Biochem., 66, 705–710 (2002). PDF This content is only available as a PDF. © Japan Society for Bioscience, Biotechnology, and Agrochemistry 2011 This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) © Japan Society for Bioscience, Biotechnology, and Agrochemistry 2011
TI - Fluorometric Determination of 2-Oxoadipic Acid, a Common Metabolite of Tryptophan and Lysine, by High-Performance Liquid Chromatography with Pre-Chemical Derivatization
JF - Bioscience Biotechnology and Biochemistry
DO - 10.1271/bbb.100723
DA - 2011-01-23
UR - https://www.deepdyve.com/lp/oxford-university-press/fluorometric-determination-of-2-oxoadipic-acid-a-common-metabolite-of-o3ltbDiD3d
SP - 185
EP - 187
VL - 75
IS - 1
DP - DeepDyve
ER -