TY - JOUR
AU - Dumas, C.
AB - Abstract During monocot embryo development, the zygote goes through a proembryo stage characterized by a radial symmetry and later becomes a true embryo with a bilateral symmetry. In order to determine culture conditions for immature embryonic stages, proembryos and embryos were isolated from controlled pollinated maize plants and developed in vitro. Precise culture conditions were determined for each type of explant: a monolayer system for embryos using NBM medium enriched with maltose (0.25 M) but without hormones, and a bilayer system for proembryo stages using N6 medium supplemented with maltose (0.35 M) and zeatin (3 mM). Morphological, cytological, and in situ hybridization analysis have shown that the resulting embryos (stages 1–2), developed in vitro, were similar to those formed in vivo and subsequently gave rise to fertile plants. This work demonstrates that successful embryo differentiation is dependent on specific parameters including the genotype, the nature of the carbon source, the type and concentration of hormones used and orientation of the embryos on the medium. The potential use of these results for embryo rescue and mutant analysis are discussed. Maize, proembryo in vitro culture, plant regeneration, ISH-Ltp2 This content is only available as a PDF. © Oxford University Press 1998 © Oxford University Press 1998
TI - In vitro development of maize immature embryos: a tool for embryogenesis analysis
JF - Journal of Experimental Botany
DO - 10.1093/jxb/49.322.839
DA - 1998-05-01
UR - https://www.deepdyve.com/lp/oxford-university-press/in-vitro-development-of-maize-immature-embryos-a-tool-for-nyZZAWwilO
SP - 839
EP - 845
VL - 49
IS - 322
DP - DeepDyve
ER -