TY - JOUR
AU1 - Coleman, James L.
AU2 - Benach, Jorge L.
AB - Patients with early Lyme disease produce IgM antibodies to a 41,000-dalton polypeptide of Borrelia burgdorferi. With advancing disease, IgO antibodies recognize a larger number of spirochetal polypeptides. Sodium dodecyl sulfate treatment of spirochetes at a concentration of 0.03% resulted in an outer envelope (OE) fraction in the supernatant and a protoplasmic cylinder (PC) flagellar fraction in the pellet. Shearing of the PC pellet followed by ultrafiltration and dialysis resulted in a flagellin-enriched fraction. From the OE and flagellin-rich fractions, individual polypeptides with Mr of 66,000, 41,000, and 31,000/34,000 daltons were eluted in microgram quantities after electrophoresis. The flagellin-enriched fraction as well as the eluted polypeptides were used as antigens in the ELISA. The flagellin-enriched fraction was superior to the standard ELISA antigen. The 41-kilodalton (kDa) flagellin antigen was not useful. Antigens of 66 kDa and 31/34 kDa were confirmed to be part of the late antibody response.
TI - Isolation of Antigenic Components from the Lyme Disease Spirochete: Their Role in Early Diagnosis
JF - The Journal of Infectious Diseases
DO - 10.1093/infdis/155.4.756
DA - 1987-04-01
UR - https://www.deepdyve.com/lp/oxford-university-press/isolation-of-antigenic-components-from-the-lyme-disease-spirochete-nyJkFunv0N
SP - 756
EP - 765
VL - 155
IS - 4
DP - DeepDyve
ER -