TY - JOUR
AU - Fidler, Isaiah J
AB - The purpose of these studies was to identify phosphatase activities required for the production of nitric oxide in murine macrophages exposed to lipopolysaccharide (LPS), synthetic lipopeptide (LPP), and mouse interferon-gamma (IFN-γ). The in vitro treatment of macrophages with IFN-γ and LPS or IFN-γ and LPP resulted in production of NO, which was inhibited by addition of the specific phosphatase 1 and 2A (PP1/2A) inhibitors okadaic acid (OA), calyculin A, and cantharidin (but not the nonactive analogues okadaic acid tetraacetate and 1,4-dimethylendothall). OA suppressed the accumulation of steady-state inducible NO synthase (iNOS) mRNA and iNOS protein (without alteration of their stability). The cytosol and nuclei of control macrophages contained large amounts of PP1/2A activities that were inhibited by OA in a dose-dependent manner. Taken together, these data indicate that PP1/2A activities are involved in the regulation of iNOS gene expression in murine macrophages.
TI - Activation of inducible nitric oxide synthase gene in murine macrophages requires protein phosphatases 1 and 2A activities
JF - Journal of Leukocyte Biology
DO - 10.1002/jlb.58.6.725
DA - 1995-12-01
UR - https://www.deepdyve.com/lp/oxford-university-press/activation-of-inducible-nitric-oxide-synthase-gene-in-murine-nugKrLi4VG
SP - 725
EP - 732
VL - 58
IS - 6
DP - DeepDyve
ER -