TY - JOUR
AU - Tampé, Robert
AB - Membrane
proteins are prime drug targets as they control the transit
of information, ions, and solutes across membranes. Here, we present
a membrane-on-nanopore platform to analyze nonelectrogenic channels
and transporters that are typically not accessible by electrophysiological
methods in a multiplexed manner. The silicon chip contains 250 000
femtoliter cavities, closed by a silicon dioxide top layer with defined
nanopores. Lipid vesicles containing membrane proteins of interest
are spread onto the nanopore-chip surface. Transport events of ligand-gated
channels were recorded at single-molecule resolution by high-parallel
fluorescence decoding.
TI - Highly Parallel Transport Recordings on a Membrane-on-Nanopore Chip at Single Molecule Resolution
JF - Nano Letters
DO - 10.1021/nl5002873
DA - 2014-02-13
UR - https://www.deepdyve.com/lp/pubmed-central/highly-parallel-transport-recordings-on-a-membrane-on-nanopore-chip-at-nkKzRvPlZh
SP - 1674
EP - 1680
VL - 14
IS - 3
DP - DeepDyve
ER -