TY - JOUR
AU - Zhao, Yan
AB - Regulation of enzyme activity is key to dynamic processes in biology but is difficult to achieve with synthetic systems. We here report molecularly imprinted nanoparticles with strong binding for the N- and C-terminal peptides on lysozyme. Binding affinity for the enzyme correlated with conformational flexibility of the peptides in the protein structure. Significantly, binding at the C-terminus of lysozyme enhanced the performance of the enzyme at elevated temperatures and that at the N-terminus lowered the enzyme activity. These nanoparticles, when clicked onto magnetic nanoparticles, could also be used to fish out the protein of interest from a mixture in a single step.
TI - Molecular recognition of enzymes and modulation of enzymatic activity by nanoparticle conformational sensors
JF - Chemical Communications
DO - 10.1039/d1cc05699a
DA - 2022-02-03
UR - https://www.deepdyve.com/lp/royal-society-of-chemistry/molecular-recognition-of-enzymes-and-modulation-of-enzymatic-activity-mmXP01wImY
SP - 1732
EP - 1735
VL - 58
IS - 11
DP - DeepDyve
ER -