TY - JOUR
AU1 - Dienes, L.
AB - PRODUCTION OF AMORPHOUS EXTRA-BACTERIAL SUB­ STANCES IN BACTERIAL CULTURES 2. OBSERVATIONS WITH VARIOUS BACTERIA, ESPECIALLY WITH GRAM­ POSITIVE AEROBIC SPORE-BEARING BACILLI L. DIENES BOSTON During the study of Oerskov's milk bacillus, we constantly looked for other bacterium strains with similar properties. The first such strain was found in a saccharose broth tube contaminated with a spore-bearing, Gram­ positive bacillus.' The broth contained, besides the bacillus, innumerable tiny non-stainable granules; and the cultures on saccharose agar plates pre­ sented a remarkable phenomenon. After some growths, every colony sur­ rounded itself with a transparent halo, similar to spreading proteus colonies; examined under the microscope, however, it was obvious that the halo did not consist of bacteria, but of a fluid-like transparent substance. The bac­ teria were well visible in the colony, but, in both the spreading and non­ spreading colonies, they stopped in a sharp line at the edge of the colony without entering the halo. Transplants from the halo could be made without difficulty; and on saccharose agar plates tiny transparent colonies were ob­ tained, in which no bacteria were visible and which did not reproduce bac­ terium colonies. The growth of this strain on agar containing no nutrient substances 
TI - Production of Amorphous Extra-Bacterial Substances in Bacterial Cultures
JF - The Journal of Infectious Diseases
DO - 10.1093/infdis/57.1.22
DA - 1935-07-01
UR - https://www.deepdyve.com/lp/oxford-university-press/production-of-amorphous-extra-bacterial-substances-in-bacterial-me01rM0mi1
SP - 22
EP - 45
VL - 57
IS - 1
DP - DeepDyve
ER -