TY - JOUR
AU - Lanir,, Naomi
AB - Abstract The double-mutant cell line 4T00.1 is derived from a plasmacytoma of a BALB/c mouse and is resistant to 6-thioguanine and ouabain. These cells were inoculated into (BALB/c × C57BL)F1 mice by different routes—sc, ip, intrasplenically, and intrathymically. The degree of tumorigenicity and pattern of tumor development were site-dependent. Intrasplenic inoculation of 103−104 4T00.1 cells resulted in development of large omental tumors accompanied by marked ascites. Tenfold to a thousandfold more 4T00.1 cells were required to obtain tumors by other routes. From all solid tumors and ascites and from various organs of tumor-bearing mice, which were explanted into culture in double selective medium containing hypoxanthine, aminopterin, and thymidine plus ouabain (in which only hybrids between 4T00.1 and normal cells can survive), proliferating and nonproliferating cultures were obtained. Of the 14 proliferating cultures, 9 proved to be hybrids by chromosome and H-2 isoantigen analyses and were tumorigenic when 106 cells were inoculated sc into syngeneic F1 mice. 2 Supported in part by the fund for promotion of research at the Technion-Israel Institute of Technology and by the Israel Cancer Association. This content is only available as a PDF. Author notes 3 Technion-Israel Institute of Technology, Faculty of Medicine, P.O. Box 9649, Haifa 31096, Israel. 4 We are grateful to Dr. John Ray, National Institutes of Health, Bethesda, Md., for the gift of mouse antisera.
TI - Fusion of Plasmacytoma and Host Cells In Vivo: Selection of Proliferating and Nonproliferating Cultures
JO - JNCI: Journal of the National Cancer Institute
DO - 10.1093/jnci/72.2.403
DA - 1984-02-01
UR - https://www.deepdyve.com/lp/oxford-university-press/fusion-of-plasmacytoma-and-host-cells-in-vivo-selection-of-md3ptEuG15
SP - 403
EP - 409
VL - 72
IS - 2
DP - DeepDyve
ER -