TY - JOUR
AU - Osterhout, Suydam
AB - The in-vitro effect of hyperbaric oxygen (HBO) on clostridia was investigated for evaluation of HBO therapy for clostridial infection. Log-phase bacteria in solid medium or broth were exposed to various pressures of O2 (usually 3 atmospheres absolute pressure); bacterial inactivation was quantitated by anaerobic incubation and viable counts. Hyperbaric oxygen was bactericidal for Clostridium perfringens. Clostridium novyi, Clostridium histolyticum, and Clostridium tetani were similarly sensitive to O2, but Clostridium bifermentans and Clostridium septicum were more resistant. Spore suspensions of C. perfringens, C. histolyticum, and C. bifermentans were resistant to 18 hr of O2 at 3 atmospheres absolute pressure. Whole blood partially blocked the inactivation of C. perfringens in HBO. The basis for this protection was the enzymatic destruction of H2O2 by catalase. Other clostridia were not protected to the same extent by catalase, suggesting that they might be more sensitive to H2O2 or to other inactivating effects of HBO.
TI - Experimental Effects of Hyperbaric Oxygen on Selected Clostridial Species. I. In-Vitro Studies
JF - The Journal of Infectious Diseases
DO - 10.1093/infdis/125.1.17
DA - 1972-01-01
UR - https://www.deepdyve.com/lp/oxford-university-press/experimental-effects-of-hyperbaric-oxygen-on-selected-clostridial-mZMG4w61BQ
SP - 17
EP - 25
VL - 125
IS - 1
DP - DeepDyve
ER -