TY - JOUR
AU - ISOI, Koichiro
AB - Abstract The uptake activity ratio for AMP, ADP, and ATP in mutant (T-1) cells of Escherichia coli W, deficient in de novo purine biosynthesis at a point between IMP and 5-aminoimidazole-4-carboxiamide-1-β-d-ribofuranoside (AICAR), was 1:0.43:0.19. This ratio was approximately equal to the 5′-nucleotidase activity ratio in E. coli W cells. The order of inhibitory effect on [2-3H]ADP uptake by T-1 cells was adenine > adenosine > AMP > ATP. About 2-fold more radioactive purine bases than purine nucleosides were detected in the cytoplasm after 5 min in an experiment with [8-14C]AMP and T-1 cells. Uptake of [2-3H]adenosine in T-1 cells was inhibited by inosine, but not in mutant (Ad-3) cells of E. coli W, which lacked adenosine deaminase and adenylosuccinate lyase. These experiments suggest that AMP, ADP, and ATP are converted mainly to adenine and hypoxanthine via adenosine and inosine before uptake into the cytoplasm by E. coli W cells. purine nucleotide, uptake, 5′-nucleotidase, purine-nucleoside phosphorylase, Escherichia coli Reference 1) Bocquet-Pages C, Lazdunski C, and Lazdunski A, Eur. J. Biochem., 118, 105–111 (1981). 2) Neu HC, J. Biol. Chem., 242, 3905–3911 (1969). 3) Kakehi M, Usuda Y, Tabira Y, and Sugimoto S, J. Mol. Microbiol. Biotechnol., 13, 96–104 (2007). 4) Yagil E and Beacham IR, J. Bacteriol., 121, 401–405 (1975). 5) Komatsu Y, Biochim. Biophys. Acta, 330, 206–221 (1973). 6) Mygind B and Munch-Petersen A, Eur. J. Biochem., 59, 365–372 (1975). 7) Zhang Y, Craig JE, and Gallagher MP, J. Bactriol., 174, 5758–5759 (1992). 8) Craig JE, Zhang Y, and Gallagher MP, Mol. Microbiol., 11, 1159–1168 (1994). 9) Xie H, Patching SG, Gallagher MP, Litherland GJ, Brough AR, Venter H, Yao SYM, Ng AML, Young JD, Herbert RB, Henderson PJF, and Baldwin SA, Mol. Membr. Biol., 21, 323–336 (2004). 10) Patching SG, Baldwin SA, Baldwin AD, Young JD, Gallagher MP, Henderson PJ, and Herbert RB, Org. Biomol. Chem., 7, 462–470 (2005). 11) Hochstadt-Ozer J, J. Biol. Chem., 247, 2419–2426 (1972). 12) Watanabe K, Fukumoto H, and Isoi K, Biochem. Biophys. Res. Commun., 134, 527–531 (1986). 13) Adelberg EA, Mandel M, and Ching Chen GC, Biochem. Biophys. Res. Commun., 18, 788–795 (1965). 14) Davis BD and Mingioli ES, J. Bacteriol., 60, 17–28 (1950). 15) Neu HC and Heppel LA, J. Biol. Chem., 240, 3685–3692 (1965). 16) Nossal NG and Heppel LA, J. Biol. Chem., 241, 3055–3062 (1966). 17) Anraku Y, J. Biol. Chem., 239, 3412–3419 (1964). 18) Kaback HR, J. Biol. Chem., 243, 3711–3724 (1968). 19) Munch-Petersen A and Pihl NJ, Proc. Natl. Acad. Sci. USA, 77, 2519–2523 (1980). 20) Norholm MHH and Dandanell G, J. Bacteriol., 183, 4900–4904 (2001). 21) Hill DL and Pittillo RF, Antimicrob. Agents Chemother., 4, 125–132 (1973). 22) Roy-Burman S and Visser DW, J. Biol. Chem., 250, 9270–9275 (1975). 23) Hochstadt-Ozer J and Stadtman ER, J. Biol. Chem., 246, 5294–5303 (1971). 24) Hochstadt-Ozer J and Stadtman ER, J. Biol. Chem., 246, 5304–5311 (1971). 25) Hochstadt-Ozer J and Stadtman ER, J. Biol. Chem., 246, 5312–5320 (1971). 26) Burton K, Biochem. J., 168, 195–204 (1977). 27) Burton K, J. Gen. Microbiol., 129, 3505–3513 (1983). 28) Andersen PS, Frees D, Fast R, and Mygind B, J. Bacteriol., 177, 2008–2013 (1995). 29) Karatza P and Frillingos S, Mol. Membr. Biol., 22, 252–261 (2005). 30) Neuhard J and Nygaard P, “Escherichia coli and Salmonella typhimuriu Cellular and Molecular Biology” Vol. 1, eds. Ingraham JL, Low KB, Neidhardt FC, Magasanik B, Schaechter M, and Umbarger HE, American Society for Microbiology, Washington DC, pp. 445–473 (1987). PDF This content is only available as a PDF. © Japan Society for Bioscience, Biotechnology, and Agrochemistry 2011 This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) © Japan Society for Bioscience, Biotechnology, and Agrochemistry 2011
TI - Uptake of AMP, ADP, and ATP in Escherichia coli W
JF - Bioscience Biotechnology and Biochemistry
DO - 10.1271/bbb.100063
DA - 2011-01-23
UR - https://www.deepdyve.com/lp/oxford-university-press/uptake-of-amp-adp-and-atp-in-escherichia-coli-w-mMaFDUXVJM
SP - 7
EP - 12
VL - 75
IS - 1
DP - DeepDyve
ER -