TY - JOUR
AU - 
AB - www.nature.com/scientificreports OPEN High‑throughput translational profiling with riboPLATE‑seq 1,2,6 1,2,3,6 4 1 Jordan B. Metz , Nicholas J. Hornstein , Sohani Das Sharma , Jeremy Worley , 1 1,5* Christian Gonzalez & Peter A. Sims Protein synthesis is dysregulated in many diseases, but we lack a systems‑level picture of how signaling molecules and RNA binding proteins interact with the translational machinery, largely due to technological limitations. Here we present riboPLATE‑seq, a scalable method for generating paired libraries of ribosome‑associated and total mRNA. As an extension of the PLATE‑seq protocol, riboPLATE‑seq utilizes barcoded primers for pooled library preparation, but additionally leverages anti‑rRNA ribosome immunoprecipitation on whole polysomes to measure ribosome association (RA). We compare RA to its analogue in ribosome profiling and RNA sequencing, translation efficiency, and demonstrate both the performance of riboPLATE‑seq and its utility in detecting translational alterations induced by specific inhibitors of protein kinases. e ce Th llular responses to many physiologic stimuli require new programs of protein production. Transcriptional regulation allows direct control of gene expression over a broad dynamic range, but cells can often more rap- idly adjust protein levels through translational control. Consequently, alongside transcription factors and their associated regulatory networks, there are post-transcriptional mechanisms 
TI - High-Throughput Translational Profiling with riboPLATE-seq
DO - 10.1101/819094
DA - 2019-10-25
UR - https://www.deepdyve.com/lp/unpaywall/high-throughput-translational-profiling-with-riboplate-seq-mCW1B9e3e1
DP - DeepDyve
ER -