TY - JOUR
AU1 - Hussain, Asif
AU2 - Shahbaz, Umar
AU3 - Khan, Salman
AU4 - Basharat, Samra
AU5 - Ahmad, Khalil
AU6 - Khan, Farhad
AU7 - Xia, Xiaole
AB - Bio-butanol, composed of four carbon hydrocarbons, is a biofuel produced from biomass feedstock by microbial activities. Butanol is used in making pharmaceutical, cosmetics, plastic, textiles, and fuel additives. Bio-production of butanol merely depends on acetone–butanol–ethanol (ABE) fermentation of clostridia at present. However, industrial butanol production with native and primitive organisms is not quantitatively and economically compatible as compared to that of fossil fuels, due to low titer, oxygen intolerance, slow growth, butanol toxicity, and by-product accumulation in the process. Therefore, metabolic engineering of microbes, including yeast, cyanobacteria, clostridial, and other bacterial species, was adopted as an approach for removing limitations associated with the process and produces a cost-effective butanol in higher amounts. In this regard, metabolic engineering strategies, such as inactivation of pathways competing with butanol by knocking out genes, introduction of engineered and more efficient enzymes in both natural and heterologous butanol producers, overexpression of genes involved in the butanol pathway, increased strain robustness to solvents, and creation of butanol pathway in non-native producers, have been applied to enable microorganisms, to enhance their capability to produce high amounts of cost-effective butanol and to reduce difficulties associated with bio-butanol synthesis such as butanol toxicity and by-product production. Therefore, we reviewed and summarized the recent advancements in metabolic engineering of microorganisms for butanol isomers (1-butanol and isobutanol) production.
TI - Advances in microbial metabolic engineering for the production of butanol isomers (isobutanol and 1-butanol) from a various biomass
JF - BioEnergy Research
DO - 10.1007/s12155-022-10410-8
DA - 2022-12-01
UR - https://www.deepdyve.com/lp/springer-journals/advances-in-microbial-metabolic-engineering-for-the-production-of-m5SQ6lrf1n
SP - 1854
EP - 1871
VL - 15
IS - 4
DP - DeepDyve
ER -